Affibody-functionalized gold-silica nanoparticles for Raman molecular imaging of the epidermal growth factor receptor |
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Authors: | Jokerst Jesse V Miao Zheng Zavaleta Cristina Cheng Zhen Gambhir Sanjiv S |
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Affiliation: | Molecular Imaging Program at Stanford, Department of Radiology, Stanford, CA 94305, USA. |
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Abstract: | The affibody functionalization of fluorescent surface-enhanced Raman scattering gold-silica nanoparticles as multimodal contrast agents for molecular imaging specific to epidermal growth factor receptor (EGFR) is reported. This nanoparticle bioconjugate reports EGFR-positive A431 tumors with a signal nearly 35-fold higher than EGFR-negative MDA-435S tumors. The low-level EGFR expression in adjacent healthy tissue is 7-fold lower than in the positive tumors. Validation via competitive inhibition reduces the signal by a factor of six, and independent measurement of EGFR via flow cytometry correlates at R(2) = 0.92. |
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Keywords: | Raman SERS nanoparticles EGFR molecular imaging |
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