大孔吸附树脂分离纯化谷胱甘肽的工艺研究

还原型谷胱甘肽(GSH)是生物体内一种具有重要生理功能的活性三肽,具有多种生理功能,发酵法是目前国内外生产GSH的主要方法。本文利用大孔吸附树脂A对GSH发酵抽提液分离纯化,结果表明:(1)大孔吸附树脂A对GSH具有良好的吸附作用,当吸附进行到150 min时,大孔吸附树脂A对GSH的吸附呈现出吸附平衡状态;(2)当pH=3.0时,大孔吸附树脂A对GSH吸附效果最佳,吸附容量和吸附率均有最大值;(3)采用动态实验的方法,对洗脱条件如洗脱剂种类、洗脱剂浓度及洗脱剂流速等进行了确定,并对其工艺过程进行了验证。在最佳实验条件下,即上柱pH=3.0,洗脱剂为0.2 mol/L的磷酸缓冲液,洗脱液流速2 mL/min,GSH抽提液的纯度可由58.5%提高到95.3%,此时洗脱液中GSH回收率>87%。

Isolation and Purification of GSH by Macroporous Resin

Reduced glutathione (GSH) is a biologically active peptide compound with a variety of physiological functions, produced mainly by fermentation. In this study, macroporous resin A was used for purification of the GSH fermentation broth. The experimental results showed that the macroporous resin A had a good adsorption on GSH. The adsorption reached equilibrium state when the process was carried out for 150 minutes. At pH 3.0, the macroporous resin A had the strongest adsorption of GSH with the maximum adsorption capacity and adsorption rate. Then, feasible pH, eluting agent species, concentration and flow rate were determined and validated by dynamic adsorption. Under the best absorption conditions (pH 3.0, 0.2 mol/L phosphate buffer as eluting agent and the eluting agent rate 2 mL/min), the purity of glutathione can be increased from 58.5% to 95.3%, and the glutathione recovery ratio in the eluting agent was > 87%, indicating that this method was effective for purification of GSH fermentation broth.