Monoclonal antibodies directed against lipopolysaccharide of Legionella pneumophila serogroup 1 |
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Authors: | AM Baranov SS Vetchinin EV Baranova SF Biketov EA Svetoch |
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Affiliation: | State Research Center for Applied Microbiology, Moscow Region, Russia. |
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Abstract: | The monoclonal antibodies (MAbs) against lipopolysaccharide of virulent strain of Legionella pneumophila serogroup 1 were produced. Three most productive hybrid clones (5F4, 5F10 and 2C9) were selected from fusions of mouse myeloma cells with spleen cells from BALB/c mice, immunized with bacterial outer membrane antigens. All generated clones were IgG-secreting. The MAbs had narrow strain specificity and showed no cross-reactions with other unrelated bacterial species. These antibodies were tested in sandwich ELISA. The results suggest that the MAbs could be used for diagnostic purposes. |
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