| PET膜表面光固定脲酶的研究 |
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| 引用本文： | 李赛,罗祥林,付皓,万昌秀,何斌.PET膜表面光固定脲酶的研究[J].高分子材料科学与工程,2001,17(2):113-115,120. |
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| 作者姓名： | 李赛 罗祥林 付皓 万昌秀 何斌 |
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| 作者单位： | 四川大学高分子材料科学与工程系, |
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| 基金项目： | 四川大学青年教师基金项目!(30 144 32 0 2 4) |
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| 摘 要： | 合成一种含芳香叠氮基的光活性酯，并利用该酯将脲酶用紫外光光固定在polyethylene terepthlalte(PET)膜的表面，X射线光电子能谱证明PET表面已经技枝脲酶，同时研究了紫外光辐照时间对固定化酶密度，固定化脲酶活性的影响,温度，pH值对游离酶，固定化酶相对活性的影响，并考察了固定化酶的相对活性随光活性酶沈度的变化及固定化酶的重复使用稳定性等性质。
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| 关 键 词： | 脲酶 固定化酶 PET膜 光固定脲酶 |
| 文章编号： | 1000-7555(2001)02-0113-03 |
THE STUDY ON THE PHOTOCHEMICAL IMMOBILIZATION OF UREASE ON THE SURFACE OF POLY(ETHYLENE TEREPHTHALATE) |
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| LI Sai,LUO Xiang-lin,FU Hao,WAN Chang-xiu,HE Bin.THE STUDY ON THE PHOTOCHEMICAL IMMOBILIZATION OF UREASE ON THE SURFACE OF POLY(ETHYLENE TEREPHTHALATE)[J].Polymer Materials Science & Engineering,2001,17(2):113-115,120. |
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| Authors: | LI Sai LUO Xiang-lin FU Hao WAN Chang-xiu HE Bin |
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| Abstract: | A novel method of using photoactivable ester with azido group was described to immobilize urease on the surface of poly(ethylene terephthalate) (PET). The effect of UV irradiation time on the immobilized urease, the influence of temperature, pH on the activity of immobilized urease was investigated. The change of the activity of the immobilized urease with the concentration of the photoactivable urease and reused times of the immobilized urease were also studied. The chemical immobilized urease was verified by ESCA. This method offered great potential to immobilize functional molecules for specific applications, such as heparin for increasing hemocompatibility, cell attachment and growth factor for cell culture applications. |
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| Keywords: | urease photochemical immobilization immobilized enzyme |
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