| 用纤维素酶-乙醇协同提取红景天总黄酮 |
| |
| 引用本文： | 毕会敏,张守勤,刘长姣.用纤维素酶-乙醇协同提取红景天总黄酮[J].吉林大学学报(工学版),2007,37(2):479-483. |
| |
| 作者姓名： | 毕会敏 张守勤 刘长姣 |
| |
| 作者单位： | 吉林大学生物与农业工程学院,长春,130022 |
| |
| 摘 要： | 以红景天为原料研究纤维素酶与乙醇溶液协同提取黄酮类物质的作用规律,考察反应条件对总黄酮浸出率的影响,并对不同提取方法的提取效果和粗提物的DPPH清除活性进行了对比。试验结果表明,纤维素酶-乙醇提取法的最佳工艺条件如下:加酶量为2.0%(以原料干重计),乙醇体积分数为36%,液料比为52∶1,pH值为5.5,反应温度为50℃,提取时间为5 h,红景天总黄酮的浸出率为7.144%。采用中等浓度的乙醇溶液作为纤维素酶的反应体系可有效提高红景天总黄酮的浸出率,粗提物得率高,且DPPH清除活性较强。
|
| 关 键 词： | 食品科学与工程 黄酮 酶解工艺 红景天 纤维素酶 DPPH清除活性 |
| 文章编号： | 1671-5497（2007）02-0479-05 |
| 收稿时间： | 2006-04-03 |
| 修稿时间： | 2006年4月3日 |
Extraction of total flavones from Rhodiola sachaIinensis by cellulase and ethanol solution |
| |
| Bi Hui-min,Zhang Shou-qin,Liu Chang-jiao.Extraction of total flavones from Rhodiola sachaIinensis by cellulase and ethanol solution[J].Journal of Jilin University:Eng and Technol Ed,2007,37(2):479-483. |
| |
| Authors: | Bi Hui-min Zhang Shou-qin Liu Chang-jiao |
| |
| Affiliation: | College of Biological and Agricultural Engineering, J ilin University, Changchun 130022, China |
| |
| Abstract: | Rhodiola sachalinensis was treated in cellulase and ethanol solution,the leaching rate of total flavones was measured under different treatment conditions.The extraction effects of different methods and the DPPH radical scavenging activities of the crude extracts were compared.Experiment results show that the optimum extraction parameters are:36% volume fraction of ethanol,2.0% dry weight of cellulase,liquid to solid ratio of 52∶1,pH 5.5,enzyme digestion temperature of 50 ℃ and extraction time of 5 h.Under such conditions the leaching rate of total flavones is up to 7.144%.The cellulase-ethanol extraction method shows high effectiveness,the crude extract has the highest yield with stronger DPPH radical scavenging activity. |
| |
| Keywords: | food science and technology flavones enzymatic process Rhodiola sachalinensis cellulase DPPH radical scavenging activity |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
