| 反胶束法提取花生蛋白后萃工艺的优化 |
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| 引用本文： | 陈茂深,朱科学.反胶束法提取花生蛋白后萃工艺的优化[J].中国油脂,2010,35(1). |
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| 作者姓名： | 陈茂深 朱科学 |
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| 作者单位： | 江南大学,食品学院,江苏,无锡,214122 |
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| 基金项目： | 国家"十一五"科技支撑计划项目资助课题 |
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| 摘 要： | 以琥珀酸二(2-乙基己基)酯磺酸钠(AOT)-异辛烷-氯化钾缓冲溶液为前萃体系,对从前萃体系中提取花生蛋白的后萃工艺条件进行了研究。考察了后萃时间、后萃温度、缓冲液pH、KCl浓度对花生蛋白后萃率的影响,并在单因素试验基础上,通过正交试验确定后萃最佳工艺条件为:后萃时间50 min,后萃温度40℃,缓冲液pH9.0,KCl浓度1.0 mol/L。在此最佳工艺条件下,花生蛋白后萃率达到86.49%。
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| 关 键 词： | 反胶束 花生蛋白 后萃 正交试验 |
Optimization of the backward extraction of protein from peanut by reverse micelles |
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| CHEN Maoshen,ZHU Kexue.Optimization of the backward extraction of protein from peanut by reverse micelles[J].China Oils and Fats,2010,35(1). |
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| Authors: | CHEN Maoshen ZHU Kexue |
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| Affiliation: | CHEN Maoshen,ZHU Kexue(School of Food Science , Technology,Jiangnan University,Wuxi 214122,Jiangsu,China) |
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| Abstract: | The backward extraction of peanut protein from forward extraction solution was studied.The reverse micellar systems were formed by sulphosuccinic acid bis(2-ethylhexyl) ester sodium salt(AOT),isooctane and KCl buffer solution.The effects of extraction time,extraction temperature,pH and KCl concentration on the backward extraction ratio were tested.On the basis of single-factor experiments,the optimum technical condition was obtained by orthogonal experiments.The results showed that the highest backward extr... |
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| Keywords: | reverse micelles peanut protein backward extraction orthogonal experiments |
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