具有3D微纳结构PDMS阵列免疫传感芯片研制

介绍了一种简便快速加工微阵列免疫传感芯片的新方法。采用化学刻蚀技术加工具有μm级山脉状起伏和nm级表面粗糙度结构(简称为3D微纳表面)的玻璃阳模,以该阳模为模板浇注法制得表面具有3D微纳表面结构的PDMS基片,再借助于物理吸附,将抗体直接固定于该PDMS表面,形成具有3D微纳结构的PDMS微阵列免疫传感器。利用光学显微镜和原子力显微镜对玻璃阳模和PDMS基片表面形貌进行表征,研究了PDMS表面微纳结构化处理对抗体吸附能力的影响。结果表明:3D微纳结构的PDMS由于具有大的比表面积,能显著增强抗体的吸附能力。将研制所得的3D微纳表面结构的PDMS芯片用于微阵列荧光免疫分析,其灵敏度是平板PDMS的5倍。

Research and fabrication of PDMS array immunosensing chip with 3D micro /nano structure

A simple and rapid method for fabrication of PDMS chip for microarray immunosensors is reported.A glass template with micro mountain-like structure and nanometer-lever roughness(termed as 3D micro /nano surface) is fabricated via chemical etching.PDMS substrates with 3D micro /nano structure are directly replicated by soft lithography against the glass template.Antibodies are immobilized onto the surface of the PDMS substrates via physical adsorption,PDMS microarray immunosensors with 3D micro /nano structure is formed.The topography of both the glass templates and PDMS substrates is characterized by optical micro spectroscope and atomic force microscope(AFM).The effect of the PDMS surface micro /nano structuring processing on the antibody-adsorbing ability is studied.It is found that antibody-adsorbing capability of the 3D micro /nano surface of PDMS substrates is significantly enhanced due to its large specific surface area.When the prepared PDMS chips with 3D micro /nano surface are applied to microarray fluorescence immunoassay,its sensitivity is 5 times of the flat PDMS chips.