新疆一枝蒿挥发油化学成分及抗氧化活性研究

用水蒸气蒸馏法从新疆一枝蒿中提取挥发油,利用GC-MS技术对其化学成分进行了分析和鉴定,并通过DPPH自由基清除体系和β-胡萝卜素漂白体系来评价新疆一枝蒿挥发油体外抗氧化作用。结果表明:共分离出49种组分,鉴定了其中的48种化合物,占总挥发油含量的89.82%,其主要成分为乙酸二氢香芹酯(31.54%)、2-羟基-3-(1-丙烯基)-1,4-萘醌(27.28%)等。抗氧化实验中,新疆一枝蒿挥发油在DPPH自由基清除体系中的IC50为0.195mg/mL,自由基清除能力相当于强抗氧化剂抗坏血酸(Vc)的70%～80%;在β-胡萝卜素漂白体系中的IC50为0.579mg/mL,抗脂质氧化能力相当于强抗氧化剂2,6-二叔丁基对甲酚(BHT)的85%～90%。表明新疆一枝蒿具有优异的抗氧化活性,且具有明显的量—效相关性。

Chemical composition and antioxidant activity of the volatile oil from the Artemisia rupestris L.

The volatile oil was extracted from Artemisia rupestris L.by steam distillation.The components of the volatile oil were separated and identified by GC-MS.The antioxidant activity was also investigated with two different methods:the 1,1-Diphenyl-2-picrylhydrazyl(DPPH) radical scavenger and the β-carotene-linoleic acid test.The results indicated that 49 kinds of components were separated and 48 components were identified among them.Accounting for about 89.82% of the total volatile oil.The main chemical constituents of the volatile oil from Artemisia rupestris L.are(-)-Dihydrocarvyl acetate(31.54%),2-hydroxy-3-(1-propenyl group)-1,4-napthoquinone(27.28%),et al.Accounting for about 63.14% of the total volatile oil.The tested IC 50 of Artemisia rupestris L.volatile oil to DPPH was 0.195 mg/mL,the equivalent of a strong free radical scavenging capacity of antioxidants ascorbic acid(Vc) of 70% to 80%;In the β-carotene-volatile oil linoleum acid system IC 50 of 0.579 mg/mL,lipid antioxidants is equivalent to strong antioxidant butylated hydroxy toluene(BHT) 85% to 90%.Therefore,Artemisia rupestris L.volatile oil had a good role in antioxidants bility.And had a concentration-effect relationship.