| 产碱性木聚糖酶菌株的选育及其酶解产物鉴定 |
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| 引用本文： | 宋玉伟,赵祥颖,董学前,杨丽萍,刘建军. 产碱性木聚糖酶菌株的选育及其酶解产物鉴定[J]. 食品科技, 2012, 0(6): 2-5,10 |
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| 作者姓名： | 宋玉伟 赵祥颖 董学前 杨丽萍 刘建军 |
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| 作者单位： | 山东轻工业学院食品与生物工程学院;山东省食品发酵工程重点实验室 |
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| 摘 要： | 以实验室保藏的1株产碱性木聚糖酶的坎皮纳斯类芽孢杆菌(P.campinasensis)xy-7为出发菌株,经紫外线、硫酸二乙酯单一及复合诱变处理,选育获得1株遗传性能稳定的高产突变株DU-36,酶活达到322IU/mL,比出发菌株提高了1倍。采用纸层析法分析,结果表明不同反应时间下酶解产物均为木二糖,推断该木聚糖酶为外切型木聚糖酶。
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| 关 键 词： | 碱性木聚糖酶 芽孢杆菌 诱变育种 低聚木糖 |
Mutation breeding of alkaline xylanase-producing strains and identification of enzymic hydrolysates |
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| SONG Yu-wei,ZHAO Xiang-ying,DONG Xue-qian,YANG Li-ping,LIU Jian-jun. Mutation breeding of alkaline xylanase-producing strains and identification of enzymic hydrolysates[J]. Food Science and Technology, 2012, 0(6): 2-5,10 |
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| Authors: | SONG Yu-wei ZHAO Xiang-ying DONG Xue-qian YANG Li-ping LIU Jian-jun |
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| Affiliation: | 1,2*(1.College of Food and Biological Engineering,Shandong Polytechnic University,Jinan 250353;2.Food & Fermentation Engineering Key Lab of Shandong Province,Jinan 250013) |
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| Abstract: | A mutant DU-36 with high alkaline xylanase activity was obtained after the strain P.campinasensis xy-7 was treated by UV-ray and diethyl sulfate.The enzyme activity reached 322 IU/mL,which increased by 100%.The result by paper chromatography indicated that the xylanase hydrolysate was always xylobiose under different hydrolysis times.From this reason,the alkaline xylanase produced by DU-36 belonged to excision enzyme. |
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| Keywords: | alkaline xylanase bacillus mutation breeding xylobiose |
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