灰毡毛忍冬茎叶不同极性成分的体外抗氧化活性研究

目的:探讨灰毡毛忍冬茎叶不同极性成分的体外抗氧化能力。方法:利用超声辅助萃取法分别得到了乙酸乙酯、正丁醇、甲醇和水提取物,采用1,1-二苯基-2-苦味偕腙肼自由基(DPPH.)法、Fe3+还原力测定实验和Folin-Ciocalteu法测总酚含量实验对各极性成分进行体外抗氧化活性评价,以Vc作阳性对照。结果表明,4种极性提取物对DPPH自由基均有较强的清除作用,其IC50值依次为14.51、6.97、8.80、11.95μg/mL;总酚含量分别达到了34.90、67.00、47.07、40.04mg/g;各提取物对铁离子的还原能力亦较强,与提取物浓度呈正相关性。结论:灰毡毛忍冬茎叶可作为天然抗氧化物质资源,具有潜在的开发利用价值。

Antioxidant activity of different polar components from the stems and leaves of Lonicera macranthoides in vitro

The aim of this study was to investigate the antioxidant activity of different polar components from the Stems and Leaves of Lonicera macranthoides in vitro.Using ultrasound-assisted extraction method the ethyl caproate,n-butanol,methanol and water extracts were obtained.The antioxidant activity of the extracts in vitro were evaluated by 1,1-diphenyl-2-picrylhydrazyl(DPPH) free radical(DPPH) system,deoxidization Fe 3+ reaction system and total phenolic content assays under the reference of vitamin C.Results showed that all the extracts can scavenge the DPPH radical effectively,the IC 50 of ethyl caproate,n-butanol,methanol and water extracts were 14.51,6.97,8.80,11.95 μg/mL;measurement of total phenolic content of the extracts were achieved using Folin-Ciocalteu reagent containing 34.90,67.00,47.07,40.04 mg/g respectively;and the extracts also possess strong reducing power.The results obtained in this study clearly indicate that the Stems and Leaves of Lonicera macranthoides have a significant potential to use as a natural antioxidant resources.