老鹰茶总皂苷的超声提取及抑菌活性研究

利用L9(34)正交设计优化了超声波提取老鹰茶总皂苷的工艺条件,并对其抑菌活性进行了研究。在单因素实验的基础上,选定超声功率、超声时间、超声温度、液料比等4个主要因素进行正交实验,确定老鹰茶总皂苷提取的最佳条件为:超声功率450W、超声时间40min、超声温度60℃、液料比30:1(mL/g),在此条件下,总皂苷含量为75.4mg/g。老鹰茶总皂苷提取物对供试的6种细菌,均呈现较强抑制作用,且提取物的浓度越高,抑菌效果越好。其中,25mg/mL的提取物对产气杆菌的抑制能力最强,抑菌圈直径为24.6mm;其次为变形杆菌和金黄色葡萄球菌;对大肠杆菌抑菌效果最弱。

Ultrasonic wave extraction and antibacterial activity of total saponins from Laoying tea

The extraction conditions of ultrasonic wave technology for total saponins from Laoying tea were optimized.Simultaneously,the antibacterial activities of total saponins extract were discussed.The optimum extraction conditions of total saponins by using orthogonal experiment based on monofactor experiments(ultrasonic wave power,extraction time,extraction temperature,and solvent-sample ratio) were obtained as follows:ultrasonic wave power was 450 W,extraction time was 40 min,extraction temperature was 60 ℃,and solvent-sample ratio was 30:1.And under the conditions,the maximum yield was 75.4 mg/g dry weight.Additionally,the total saponins extract had different inhibition effect on six kinds of bacteria,and showed the dose-dependent manner.And at the concentration of 25 mg/mL,the inhibition ability on Enterobacter aerogenes was the strongest and its inhibiting zone diameter was 24.6 mm,then was Proteusbacillus vulgaris and Staphylococcus aureus,the weakest was on Escherichia coli.