| 中国林蛙皮中胶原蛋白的提取研究 |
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| 引用本文： | 李琳琳,鲍成伟,杨翔华,王战勇. 中国林蛙皮中胶原蛋白的提取研究[J]. 食品科技, 2012, 0(2): 265-268 |
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| 作者姓名： | 李琳琳 鲍成伟 杨翔华 王战勇 |
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| 作者单位： | 辽宁石油化工大学环境与生物工程学院 |
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| 摘 要： | 采用酶解法从中国林蛙皮中提取胶原蛋白。考查了提取次数、液料比、酶解时间、酶解温度以及酶用量对胶原蛋白提取率的影响。单因素考察结合正交试验设计得到胶原蛋白的优化提取条件为:提取次数为2次,液料比为60,提取时间为72 h,提取温度为37℃,酶用量为1.5%。此条件下中国林蛙皮胶原蛋白的产率可达26.2%。
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| 关 键 词： | 中国林蛙 胶原蛋白 提取 纯化 |
Preparation of collagen from the skin of Rana chensinensis |
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| LI Lin-lin,BAO Cheng-wei,YANG Xiang-hua,WANG Zhan-yong. Preparation of collagen from the skin of Rana chensinensis[J]. Food Science and Technology, 2012, 0(2): 265-268 |
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| Authors: | LI Lin-lin BAO Cheng-wei YANG Xiang-hua WANG Zhan-yong |
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| Affiliation: | *(School of Environmental and Biological Engineering,Liaoning Shihua University, Fushun 113001) |
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| Abstract: | The skins of Rana chensinensis were used to preparation of collagen.The experiment examines ratio of liquids to raw material,reaction time,reaction temperature and enzyme dosage on the extraction rate of collagen.Combining single factor study and orthogonal test,the optimal extraction conditions were chosen as follows: extraction number is 2,extraction time is 72 h,extraction temperature is 37 ℃,enzyme dosage is 1.5%,ratio of liquids to raw material is 60.Under these conditions,the yield of Rana chensinensis skin collagen is 26.2%. |
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| Keywords: | Rana Chensinensis collagen extraction purification |
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