| 金针菇漆酶活性测定的最佳反应条件及液体培养胞外酶的研究 |
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| 引用本文： | 刘苹,唐志红,李梦玉,温少红. 金针菇漆酶活性测定的最佳反应条件及液体培养胞外酶的研究[J]. 食品科技, 2012, 0(6): 14-17 |
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| 作者姓名： | 刘苹 唐志红 李梦玉 温少红 |
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| 作者单位： | 烟台大学生命科学学院 |
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| 基金项目： | 山东省优秀中青年科学家科研奖励基金项目(2008BS02009);山东省科技攻关计划项目(2010GGX10721) |
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| 摘 要： | 研究了ABTS[(2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)]法测定金针菇漆酶活性的最佳反应条件,确定了显色反应的最佳条件为:适宜测定波长为410nm、体系pH4.0、反应温度40℃。对金针菇LP03液体培养过程中的胞外酶活性进行检测,研究了漆酶、纤维素酶、木聚糖酶、过氧化物酶、锰过氧化物酶和淀粉酶的变化规律。在整个培养过程中这6种酶的活性存在很大差异。
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| 关 键 词： | 漆酶 ABTS 金针菇 胞外酶 |
Optimum reaction conditions on laccase activity determination and extracellular enzymes during liquid culture in Flammulina velutipes |
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| LIU Ping,TANG Zhi-hong,LI Meng-yu,WEN Shao-hong. Optimum reaction conditions on laccase activity determination and extracellular enzymes during liquid culture in Flammulina velutipes[J]. Food Science and Technology, 2012, 0(6): 14-17 |
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| Authors: | LIU Ping TANG Zhi-hong LI Meng-yu WEN Shao-hong |
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| Affiliation: | *(College of Life Science,Yantai University,Yantai 264005) |
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| Abstract: | The optimum conditions of laccase activity determination by ABTS[2,2’-Azino-bis(3-ethylbenzt hiazoline-6-sulfonic acid)] in the liquid culture of Flammulina velutipes LP03 were studied.The wave length,pH and temperature were 410 nm,4.0 and 40 ℃,respectively.The activity of laccase,cellulase,xylanase,peroxidase,manganese peroxidase and amylase was detected in the culture.The activity of six enzymes differed greatly. |
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| Keywords: | laccase ABTS Flammulina velutipes LP03 extracellular enzymes |
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