木瓜蛋白酶制备草鱼鳞胶原肽的工艺优化及产物特性分析

以草鱼鱼鳞为对象,研究木瓜蛋白酶酶解条件对鱼鳞蛋白酶解产物水解度和氮收率的影响,以获得较高氮收率及水解度的鱼鳞胶原肽。结果表明,水解温度、起始pH值、底物浓度和加酶量对木瓜蛋白酶水解产物的水解度和氮收率都有显著影响(p<0.05);木瓜蛋白酶在底物浓度为5%、起始pH值为5.0、加酶量为3000 U/g蛋白、水解温度为60℃条件下水解鱼鳞120 min,鱼鳞水解产物的水解度和氮收率都较好,其水解度为12.68%、氮收率为89.42%;且水解产物在酸性、中性及碱性条件下的溶解性均在97%以上,分子量呈连续分布,主要分布在200～ 5000 u之间。

Processing optimization and properties of collagen peptide extraction from grass carp scales with papain

The effects of hydrolyzing conditions on the enzymatic hydrolysis of grass carp scales with papain and the hydrolyzing process optimized by orthogonal experiment were studied.There was a significant effect of hydrolyzing temperature,initial pH value,substrate concentration,and papain dosage on the hydrolyzing degree and nitrogen recovery of papain hydrolysates(p<0.05).Hydrolyzing temperature of 60 ℃,initial pH value 5.0,papain dosage of 3000 U/g Pro,substrate concentration 5%(w/v),and a hydrolysis time of 120 min were found to be the optimum conditions to obtain a higher nitrogen recovery and hydrolyzing degree,and the hydrolyzing degree and nitrogen recovery of papain hydrolysate were 12.68% and 89.42%,respectively.The solubility of collagen peptide was above 97% in acidic,neutral and alkaline conditions and molecular weight mainly distributed in 200~5000 u by analyzing physiochemical properties of hydrolysates.