| 离子液体萃取/HPLC测定水产品中司帕沙星残留研究 |
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| 引用本文： | 万雄,郁迪,董洁莹,孙俏洁,盛寒玉,杨晓露,季龙娟,金丽华. 离子液体萃取/HPLC测定水产品中司帕沙星残留研究[J]. 食品科技, 2012, 0(5): 303-306 |
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| 作者姓名： | 万雄 郁迪 董洁莹 孙俏洁 盛寒玉 杨晓露 季龙娟 金丽华 |
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| 作者单位： | 浙江海洋学院食品与药学学院;佳木斯大学药学院 |
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| 基金项目： | 浙江省自然科学基金项目(Z3100533,Y2111223);浙江省科技厅公益计划项目(2011C22073);浙江省新苗计划项目(2010R411012) |
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| 摘 要： | 建立了以离子液体([HMIm]BF4)为萃取剂,HPLC检测水产品中残留司帕沙星的方法,采用反相SpherisorbC18色谱柱(250mm×4mmi.d.,5μm),流动相为甲醇,流速:0.5mL/min,检测波长:298nm。在0.06～0.18mg/mL浓度范围内,方法线性良好(R2=0.9988),回收率为88.7%～93.0%,日内和日间的RSD介于2.1%～3.7%。方法准确可靠,重现性好,灵敏度高,适用于水产品中残留药物的检测。
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| 关 键 词： | 离子液体 高效液相色谱 水产品 司帕沙星 |
Application of ionic liquid as extraction agent and determination of sparfloxacin in the aquatic products by HPLC |
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| WAN Xiong,YU Di,DONG Jie-ying,SUN Qiao-jie,SHENG Han-yu,YANG Xiao-lu,JI Long-juan,JIN Li-hua. Application of ionic liquid as extraction agent and determination of sparfloxacin in the aquatic products by HPLC[J]. Food Science and Technology, 2012, 0(5): 303-306 |
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| Authors: | WAN Xiong YU Di DONG Jie-ying SUN Qiao-jie SHENG Han-yu YANG Xiao-lu JI Long-juan JIN Li-hua |
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| Affiliation: | 1(1.School of Food and Pharmacy,Zhejiang Ocean University,Zhoushan 316000;2.College of Pharmacy,Jiamusi University,Jiamusi 154007) |
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| Abstract: | Determination of sparfloxacin in the aquatic products using [HMIm] BF4 as extraction agent coupled with HPLC was investigated.The column configuration consisted of Spherisorb C18 column(250 mm×4 mm i.d.,5 μm particle size).The mobile phase was methanol at a flow rate 0.5 mL/min,the UV detector was set at 298 nm.calibration curve was linear over the concentration ranges tested with 0.06～0.18 mg/mL(R2=0.9988).The precision(RSD%) and accuracy were 2.1～3.7 and 88.7%～93.0%,respectively.The method was validated for linearity,precision and intra-and inter-day variation,and was suitable to the determination of drug residues in aquatic products. |
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| Keywords: | ionic liquid high performance liquid chromatography aquatic products sparfloxacin |
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