响应面法优化米曲霉产AMP脱氨酶的培养基

采用响应面方法对米曲霉产AMP脱氨酶的发酵培养基进行优化。首先通过Plackett-Burman实验,筛选出3个主要的影响因素:蛋白胨,MgSO4.7H2O和吐温-80。然后运用爬坡路径法对这3种因子进行实验,获得这3种重要因子的最适质量浓度范围。最后通过响应面分析法,得出3种重要影响因子的交互作用及最佳条件。确定米曲霉产AMP脱氨酶的最佳发酵培养基为:葡萄糖3%,蛋白胨2.52%,三水柠檬酸二钠0.4%,MgSO4.7H2O0.04%,微量元素母液4%,吐温-800.16%,在此最佳培养基下发酵酶活可达361.33U/mL,比优化前提高了29.05%。

Optimization of medium components for AMP deaminase production by Aspergillus oryzae using response surface methodology

The fermentation medium components for AMP deaminase production by Aspergillus oryzae was optimized by response surface methodology(RSM).Firstly,the three of the most important influence factors peptone,MgSO4.7H2O and Tween-80 was obtained through Plackett-Burman design screening.Then the path of steepest ascent experiment was adopted to approach the optimal region of the medium composition.Finally,the optimal concentration and mutual effect of three factors were obtained by RSM.The results showed that the best medium composition was glucose 3%,peptone 2.52%,trisodium citrate dehydrate 0.4%,MgSO4.7H2O 0.04%,trace elements liquor 0.4%,Tween-80 0.16%.Under the above optimum fermentation conditions the enzyme activity of AMP deaminase can be enhanced to 361.33 U/mL,increasing 29.05% than before optimization.