| 具有胆盐水解酶活性乳酸菌的筛选及影响其活性因素分析 |
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| 引用本文： | 王俊国,包秋华,陈永福,刘文俊,丹彤,张和平.具有胆盐水解酶活性乳酸菌的筛选及影响其活性因素分析[J].食品科技,2012(8):8-12. |
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| 作者姓名： | 王俊国 包秋华 陈永福 刘文俊 丹彤 张和平 |
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| 作者单位： | 内蒙古农业大学食品科学与工程学院乳品生物技术与工程教育部重点实验室 |
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| 基金项目： | 国家自然科学基金项目(30840011);中国科学院西部之光人才培养计划项目;内蒙古农业大学博士启动基金项目(BJ08-18);内蒙古自然科学基金项目(2011MS1205) |
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| 摘 要： | 具有胆盐水解酶活性的乳酸菌有潜在的降低血液胆固醇的作用。对分离自酸马奶的10株乳杆菌的胆盐水解酶活性进行了定性及定量分析,在此基础上对影响胆盐水解酶的一些因素进行了研究。结果表明,3株乳杆菌具有胆盐水解酶活性,其中以MG13-3为最高,其次为XB4-1及MG16-3;当pH值为6时,超声波破碎时间在12～15min之间,培养基中加入巯基乙酸钠并在厌氧条件下培养时,3株乳杆菌均体现出较高的胆盐水解酶活性。
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| 关 键 词： | 胆盐水解酶 乳酸菌 影响因素 |
Screening of potential bile salt hydrolase activity of lactobacilli and analysis of some influence factors on activity of BSH |
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| WANG Jun-guo, BAO Qiu-hua, CHEN Yong-fu, LIU Wen-jun, DAN Tong, ZHANG He-ping.Screening of potential bile salt hydrolase activity of lactobacilli and analysis of some influence factors on activity of BSH[J].Food Science and Technology,2012(8):8-12. |
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| Authors: | WANG Jun-guo BAO Qiu-hua CHEN Yong-fu LIU Wen-jun DAN Tong ZHANG He-ping |
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| Affiliation: | * (Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Huhhot 010018) |
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| Abstract: | Lactic acid bacteria with bile salt hydrolase(BSH) activity has potential importance in reducing serum cholesterol. In this study, ten strains of lactobacilli isolated from the koumiss were tested for their bile salt hydrolase activity. The environment factors, ultrasonic cytoclasis time which influenced the activity of bile salt hydrolase were studied. Three strains of lactobacillus with BSH activity were screened out. For the activity of bile salt hydrolase, the optimum pH was 6 and optimum ultrasonic cytoclasis time was 12 to 15 min for all three strains. Meanwhile, Addition of 0.2% THIO and anaerobic environment can improve BSH activity of three strains. |
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| Keywords: | bile salt hydrolase lactobacilli influence factors |
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