| 基因组改组选育低色素高产茁霉多糖生产菌株 |
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| 引用本文： | 张晶,王丹,张静,王玉华. 基因组改组选育低色素高产茁霉多糖生产菌株[J]. 食品科技, 2012, 0(7): 16-21 |
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| 作者姓名： | 张晶 王丹 张静 王玉华 |
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| 作者单位： | 吉林农业大学食品科学与工程学院 |
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| 基金项目： | 吉林省科技厅科技支撑计划项目(20080251) |
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| 摘 要： | 以出芽短梗霉(Aureobasidium pullulans)高产菌P1、P2和低色素菌P3、P4为出发菌,通过5轮基因组改组,摇瓶发酵复筛选出2株产色素能力低、多糖产量较高的菌株F51、F52,其中菌株F51、F52多糖产量分别为42.38、42.60g/L,比出发菌株P1多糖产量提高41.27%、42.00%,发酵液OD值为0.378、0.363,比出发菌P3降低17.30%、20.57%。
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| 关 键 词： | 基因组改组 出芽短梗霉 茁霉多糖 筛选 |
Breeding of low-pigment and high-yield pullulan strain by genome shuffling |
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| ZHANG Jing,WANG Dan,ZHANG Jing,WANG Yu-hua. Breeding of low-pigment and high-yield pullulan strain by genome shuffling[J]. Food Science and Technology, 2012, 0(7): 16-21 |
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| Authors: | ZHANG Jing WANG Dan ZHANG Jing WANG Yu-hua |
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| Affiliation: | *(College of Food Science and Engineering,Jilin Agricultural University,Changchun 130118) |
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| Abstract: | Aureobasidium pullulans P1,P2,P3 and P4 were treated by five wheels genome shuffling,then screened two low-pigment and high-yield strains F51 and F52 by fermentation test.The polysaccharide yield of shuffled strains F51,F52 were 42.38 g/L,42.60 g/L,improved 41.27%,42.00% than the original strain P1,respectively.The fermentation broth OD of F51,F52 were 0.378,0.363,decreased 17.30%,20.57% than the original strain P3,respectively. |
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| Keywords: | genome shuffling Aureobasidium pullulans pullulan screening |
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