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甘蔗叶中黄酮类化合物的提取工艺研究
引用本文:冯纪南,赵丽萍,黄海英,章爱华,邓斌. 甘蔗叶中黄酮类化合物的提取工艺研究[J]. 食品科技, 2012, 0(4): 219-223
作者姓名:冯纪南  赵丽萍  黄海英  章爱华  邓斌
作者单位:湘南学院化学与生命科学系;湘南学院教务处;吉首大学化学化工学院
基金项目:湖南省科技厅计划项目(2010GK3123);湖南省教育厅重点科研基金项目(10A113)
摘    要:采用分光光度法,以芦丁为标准品测定甘蔗叶中黄酮类化合物含量。在提取过程中通过单因素试验分析了乙醇浓度、料液比、浸取温度与浸取时间4个主要因素对甘蔗叶中黄酮类化合物提取率的影响。并在单因素试验的基础上通过正交实验优化了甘蔗叶中黄酮类化合物提取工艺条件。结果表明:甘蔗叶黄酮类化合物的最佳提取工艺条件为乙醇体积浓度为70%,料液比为1:40(g/mL),浸取时间为3.0h,浸取温度为70℃,此条件下甘蔗叶中黄酮类化合物的提取率可达5.81%。

关 键 词:甘蔗叶  黄酮类化合物  提取

Extraction technology of flavonoides from sugar cane leaves
FENG Ji-nan,ZHAO Li-ping,HUANG Hai-ying,ZHANG Ai-hua,DENG Bin. Extraction technology of flavonoides from sugar cane leaves[J]. Food Science and Technology, 2012, 0(4): 219-223
Authors:FENG Ji-nan  ZHAO Li-ping  HUANG Hai-ying  ZHANG Ai-hua  DENG Bin
Affiliation:1(1.Department of Chemistry and Life Science,Xiangnan University,Chenzhou 423000;2.Education Affair Office,Xiangnan University,Chenzhou 423000;3.College of Chemistry and Chemical Engineering,Jishou University,Jishou 416000)
Abstract:Flavonoides content in sugar cane leaves was detected by spectrometric method with rutin as standard material.In the extracting process of flavonoides,four main factors including concentration of extracting solution,solid-liquid ratio,extracting temperature and time were studied by single factor analysis method.The results showed that the optimal extracting conditions obtained by orthogonal analysis were as follows:ethanol concentration 70%,solid-liquid ratio 1:40(g/mL),extracting duration 3.0 h and refluxing temperature 70 ℃.Under these optimum experimental conditions,flavonoides yield from sugar cane leaves could reach 5.81%.
Keywords:sugar cane leaves  flavonoides  extraction
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