树脂分离纯化萌发糙米中多酚的研究

比较了AB-8、X-5、NKA、NKA-2、S-85种大孔树脂对萌发糙米多酚的吸附和解吸性能,结果表明,AB-8树脂具有较好的吸附性能和解吸效果;确定了AB-8树脂分离萌发糙米多酚的适宜操作条件为:上柱料液浓度为0.4716mg/mL,流速为2.0mL/min,以蒸馏水和浓度为70%乙醇进行洗脱,解吸速率为2.0mL/min,得到萌发糙米多酚纯度为63.25%。AB-8树脂可用于萌发糙米多酚的分离纯化。

Isolation and purification of polyphenols in pre-germinated brown rice by macroporous resin

Adsorption capacity and static desorption rate of adsorption resins such as AB-8,X-5,NKA,NKA-2,S-8 for polyphenols of pre-germinated brown rice were assayed.AB-8 resin had the best adsorption capacity and static desorption rate for polyphenols.The optimal conditions of isolation and purification for polyphenols of pre-germinated brown rice by AB-8 resin were obtained.The crude polyphenols extract solution with the concentration of 0.4716 mg/mL was first subjected to AB-8 resin adsorption at the flow rate of 2.0 mL/min.Then the impurities were eluted down by distilled water and 70% ethanol at the desorption rate of 2.0 mL/min.The purity of resulting polyphenols of pre-germinated brown rice was 63.25%.The polyphenols of pre-germinated brown rice could be isolated and purified by AB-8 resin.