无菌钝顶螺旋藻单细胞的制备和再生

用含有0．75mol/LNaCl的Zarrouk培养基洗去藻丝体表面的角质鞘，可获得大量有活性并能再生的单细胞，再生率为28．6％。用0．4％的次氯酸钠溶液消毒藻丝体5分钟，恢复培养后再置于含有终浓度均为150μg/μl的卡那霉素、庆大霉素和新霉素Zarrouk培养基中35℃48h杀菌，最后用含1．5mol/LNaCl的Zarrouk培养基对藻丝体洗壁处理1min，可得无菌螺旋藻品系。

Preparation and Regeneration of Single Cells from Axenic Spirulina platensis

The present study suggested a lysozyme-free method for preparation of single cells of axenic Spirulina platensis.When the filaments of Spirulina platensis washed by Zarrouk medium containing 0.75mol/L NaCl,many single cdlls were obtained and the regeneration rate of single cells was 28.6%.A method is also explored for preparation of axenic strain of Spirulina platensis.Firstly,the filaments were treated with 0.4% NaClO for 5 minutes,secondly,put the filaments into the Zarrouk medium containing Kanamycin,Gentamicin and Neomycin,the final concentration of these three antibiotic is 15.μg/μl,cultured the filaments under 35℃ for 48 hours,finally the filaments were rinsed with Zarrouk medium containing 1.5mol/L NaCl for 1minute.