| 广西香港海鸥菌分离菌株脉冲场凝胶电泳分子分型研究 |
| |
| 引用本文： | 孙贵娟,黄彦,唐振柱,卢桂宁,苏伟东,甘永新. 广西香港海鸥菌分离菌株脉冲场凝胶电泳分子分型研究[J]. 中国食品卫生杂志, 2011, 23(6): 494-496 |
| |
| 作者姓名： | 孙贵娟 黄彦 唐振柱 卢桂宁 苏伟东 甘永新 |
| |
| 作者单位： | 广西壮族自治区疾病预防控制中心,广西 南宁,530028 |
| |
| 摘 要： | 目的 探讨香港海鸥菌分子分型方法,了解广西水产品监测所分离的香港海鸥菌的相关性.方法 以NotⅠ限制性内切酶对2005年分离的香港海鸥菌酶切后进行脉冲电泳,用BioNumerics 5.1聚类分析获得电泳图谱.结果 7株香港海鸥菌分为6个分子型,其中从南宁分离的与从河池分离的2株香港海鸥菌高度同源,相似度达100％.结论 PFGE可应用于香港海鸥菌分子分型,有助于发现香港海鸥菌流行规律和传播途径,水鸟可能是香港海鸥菌传播环节的一种重要媒介.
|
| 关 键 词： | 香港海鸥菌 脉冲场凝胶电泳 分子分型 食源性病原菌 媒介 |
| 收稿时间： | 2011-04-19 |
Molecular typing of Laribacter hongkonggensis isolated in Guangxi province by pulsed-field gel electrophoresis |
| |
| Sun Guijuan,Huang Yan,Tang Zhenzhu,Lu Guining,Su weidong and Gan Yongxin. Molecular typing of Laribacter hongkonggensis isolated in Guangxi province by pulsed-field gel electrophoresis[J]. Chinese Journal of Food Hygiene, 2011, 23(6): 494-496 |
| |
| Authors: | Sun Guijuan Huang Yan Tang Zhenzhu Lu Guining Su weidong Gan Yongxin |
| |
| Affiliation: | Sun Guijuan,Huang Yan,Tang Zhenzhu,Lu Guining,Su weidong,Gan Yongxin(Guangxi Zhuang Autonomous Region Center for Disease Prevention and Control,Guangxi Nanning 530028,China) |
| |
| Abstract: | Objective To study the molecular typing of Laribacter hongkonggensis by pulsed-field gel electrophoresis(PFGE),and to find out the relationship of 7 strains of L.hongkonggensis isolated from fishery products in Guangxi on 2005.Methods Total DNA of each isolate was digested with restriction enzyme Not I,and the DNA fingerprints were obtained by running PFGE.These fingerprints of different stains were compared and their relationship was investigated by BioNumerics 5.1 clustering.Results Seven strains of L.hon... |
| |
| Keywords: | Laribacter hongkonggensis pulsed-field gel electrophoresis(PFGE) molecular typing food-born pathogenic medium |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国食品卫生杂志》浏览原始摘要信息 |
|
点击此处可从《中国食品卫生杂志》下载全文 |
|
