| 祈州漏芦的组织培养与快繁技术研究 |
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| 引用本文： | 赵春艳,刘金成,方坤,王宏波.祈州漏芦的组织培养与快繁技术研究[J].中国食物与营养,2011,17(10):28-29. |
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| 作者姓名： | 赵春艳 刘金成 方坤 王宏波 |
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| 作者单位： | 1. 哈尔滨医科大学大庆校区,黑龙江大庆,165519
2. 哈尔滨医科大学附属五院,黑龙江大庆,163319 |
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| 基金项目： | 黑龙江省教育厅科学技术研究项目“大庆地区祁州漏芦资源的开发利用” |
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| 摘 要： | 目的：对祁州漏芦愈伤组织诱导培养基和生长的培养条件进行研究,构建祁州漏芦快速繁殖体系。方法：通过筛选外植体,添加不同种类及浓度的生长调节剂对祁州漏芦进行愈伤组织诱导、芽增殖培养及生根培养。结果：诱导愈伤组织适宜培养基：MS＋0.3mg/L NAA＋3.0mg/L 6-BA;芽增殖培养以MS＋0.3mg/L NAA＋5.0mg/L 6-BA为宜;生根培养以1/2MS＋0.1mg/L NAA为佳。结论：利用组织培养技术能够实现祁州漏芦快速繁殖。
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| 关 键 词： | 祁州漏芦 组织培养 快速繁殖 |
Tissue Culture and Rapid Propagation of Rhaponticum uniflorum |
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| ZHAO Chun-yan,LIU Jin-cheng,FANG Kun,WANG Hong-bo.Tissue Culture and Rapid Propagation of Rhaponticum uniflorum[J].Food and Nutrition in China,2011,17(10):28-29. |
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| Authors: | ZHAO Chun-yan LIU Jin-cheng FANG Kun WANG Hong-bo |
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| Affiliation: | ZHAO Chun-yan1,LIU Jin-cheng1,FANG Kun1,WANG Hong-bo2 (1 Harbin Medical University Daqing Campus,Daqing 163319,China,2 Five Affiliated Hospital of Harbin Medical University,China) |
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| Abstract: | 【Objective】 To provide some new evidences for rapid propagation of Rhaponticum？ uniflorum through studying on medium with callus tissue culture and the condition of growth.【Method】 Callus tissue induction,bug proliferation culture,rooting culture of Rhaponticum uniflorum were conducted by explants selecting and growth regulator adding different hormones.【Result】 The appropriate medium for different culture stages were MS＋0.3mg/L NAA＋3.0mg/L 6-BA in callus culture induction,MS＋0.3mg/L NAA＋5.0mg/L 6-BA in differentiation and subculture of cluster buds,1/2MS＋0.1mg/L NAA in rooting culture.【Conclusion】Rapid propagation can be achieved with plant tissue culture in Rhaponticum uniflorum. |
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| Keywords: | Rhaponticum uniflorum tissue culture rapid propagation |
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