| 动物性食品中盐酸克伦特罗ELISA检测方法的建立及应用 |
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| 引用本文： | 周群标,桑亚新,王丽,李星,王向红. 动物性食品中盐酸克伦特罗ELISA检测方法的建立及应用[J]. 中国食品学报, 2011, 11(6) |
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| 作者姓名： | 周群标 桑亚新 王丽 李星 王向红 |
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| 作者单位： | 河北农业大学食品科技学院,河北省农产品加工工程技术研究中心,河北保定071001 |
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| 基金项目： | 国家海洋局公益性行业科研专项(No.200805046) |
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| 摘 要： | 目的:建立动物性食品中盐酸克伦特罗残留快速检测技术,确保食品安全.方法:以盐酸克伦特罗重氮化后分别连接到牛血清蛋白(BSA)和卵清蛋白(OVA)上制得免疫原BSA-CL和包被抗原OVA-CL.通过免疫得到多抗血清,经硫酸铵沉淀,Sephrose 4B-proteinA对抗体进行纯化,在此基础上建立间接竞争ELISA方法.结果表明:包被抗原的最适质量浓度为10 μg/mL,抗体的最佳稀释度为1∶1000;该方法的IC50为2.18 ng/mL,最低检测限达0.05 ng/mL,与沙丁胺醇和妥洛特罗的交叉反应率分别为8.28％和7.75％,平均加标回收率为90.78％.结论:该法灵敏度高,样品前处理简便、快速,适用于动物源性食品中盐酸克伦特罗残留的大批量现场检测.
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| 关 键 词： | 盐酸克伦特罗 ELISA 检测方法 |
Establishment and Application of ELISA Method for Detection of Clenbuterol Hydrochloride in Animal Foods |
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| Zhou Qunbiao,Sang Yaxin,Wang Li,Li Xing,Wang Xianghong. Establishment and Application of ELISA Method for Detection of Clenbuterol Hydrochloride in Animal Foods[J]. Journal of Chinese Institute of Food Science and Technology, 2011, 11(6) |
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| Authors: | Zhou Qunbiao Sang Yaxin Wang Li Li Xing Wang Xianghong |
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| Affiliation: | Zhou Qunbiao Sang Yaxin Wang Li Li Xing Wang Xianghong*(Engineering Research Center of agricultural products' processing in Hebei Province,College of Food Science & Technical,Agricultural University of Hebei,Baoding 071001,Hebei) |
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| Abstract: | Objective:An indirect enzyme-linked immunosorbent assay(ELISA) was developed to detect Clenbuterol hydrochloride in animal foods in order to ensure food safety.Methed:Hapten clenbuterol was conjugated with Bovine Sera Albumin(BSA) and Ovalbumin(OVA) by diazotization to produce immunogen BSA-CL and OVA-CL used for immunizing rabbit.The rabbit antiserum was purified with ammonium sulfate fractionation to prepare polyclonal antibody against clenbuterol hydrochloride.An indirect enzyme-linked immunosorbent assa... |
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| Keywords: | clenbuterol hydrochloride enzyme-linked immunosorbent assay method validation |
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