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| PCR扩增法鉴定年糕中腐败细菌的初步研究 | |
| 引用本文： | 刘青梅,喻勇新,张玲,杨性民. PCR扩增法鉴定年糕中腐败细菌的初步研究[J]. 中国食品学报, 2011, 11(5) |
| 作者姓名： | 刘青梅 喻勇新 张玲 杨性民 |
| 作者单位： | 1. 浙江万里学院宁波市农产品加工技术重点实验室 浙江宁波315100 2. 上海海洋大学食品学院 上海201306 3. 浙江万里学院宁波市农产品加工技术重点实验室 浙江宁波315100;上海海洋大学食品学院上海201306 |
| 基金项目： | 浙江省科技厅2005年农业攻关项目(2005SA96001) |
| 摘 要： | 采用平板划线分离方法,从腐败年糕中分离得到疑似腐败菌.对分离、纯化培养获得的7株疑似菌进行16S rDNA基因序列测定及系统发育树分析等方面的分子鉴定,结果表明7株菌分为芽孢杆菌和短芽孢杆菌(RC1)2属.其中芽孢杆菌属中的6株菌又分为解淀粉芽孢杆菌(RC7)、芽孢杆菌(RC2)和枯草芽孢杆菌(RC3、RC4、RC5、RC6)3个种. |
| 关 键 词： | PCR扩增 鉴定 年糕 腐败菌 |
Preliminary Study on Identification of Spoilage Bacteria in Rice Cakes by PCR Amplification Method |
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| Liu Qingmei,Yu Yongxin,Zhang Ling,Yang Xingming. Preliminary Study on Identification of Spoilage Bacteria in Rice Cakes by PCR Amplification Method[J]. Journal of Chinese Institute of Food Science and Technology, 2011, 11(5) | |
| Authors: | Liu Qingmei Yu Yongxin Zhang Ling Yang Xingming |
| Affiliation: | Liu Qingmei1 Yu Yongxin2 Zhang Ling1,2 Yang Xingming1(1Zhejiang Wanli University,Ningbo Key Lab of Agriculture Product Processing Technology,Ningbo 315100,Zhejiang 2College of Food Science and Technology,Shanghai Ocean University.,Shanghai 201306) |
| Abstract: | Spoilage bacteria in the rice cakes were isolated by the streak plate separation method.Seven bacteria were purified and cultivated for molecular identification by 16S rDNA gene sequencing detection and phylogenetic tree analysis.The result showed that these strains can be classified as two genera,of Bacillus and Brevibacillus in which the 6 strains in Bacillus also could be classified as three species of Bacillus amyloliquefaciens(RC7),Bacillus velezensis(RC2)and Brevibacillus agri(RC3,RC4,RC5,RC6). |
| Keywords: | PCR amplification identification rice cakes spoilage bacteria |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |