| 微波辅助提取孔鳐抗氧化和血管生成抑制活性软骨多糖的研究 |
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| 引用本文： | 罗红宇,王斌,冯刚,曲有乐. 微波辅助提取孔鳐抗氧化和血管生成抑制活性软骨多糖的研究[J]. 中国食品学报, 2011, 11(7) |
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| 作者姓名： | 罗红宇 王斌 冯刚 曲有乐 |
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| 作者单位： | 1. 华中科技大学生命科学与技术学院 武汉 430074;浙江海洋学院食品与药学学院 浙江舟山 316004
2. 浙江海洋学院食品与药学学院 浙江舟山 316004 |
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| 基金项目： | 国家自然科学基金，浙江省自然科学基金 |
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| 摘 要： | 目的:对微波辅助碱液提取孔鳐软骨多糖的方法及其抗氧化和血管生成抑制活性进行研究.方法:采用微波辅助碱液提取与木瓜蛋白酶水解相结合的加工工艺,以正交试验法优化提取工艺参数.通过还原力、DPPH·和·OH清除实验对多糖抗氧化能力进行测定.采用鸡胚绒毛尿囊膜(CAM)模型对其血管生成抑制活性进行评价.结果:微波辅助碱液提取最佳工艺参数是:微波功率650W、提取温度60℃、碱质量分数15％、液料比35:1、提取时间7min.木瓜蛋白酶脱蛋白精制孔鳐软骨多糖的最佳条件是:酶解温度50℃,pH 7.0,酶解时间2h,酶量1.0％.在此条件下精制的多糖为白色粉末,得率13.77％,纯度88.75％.在相同浓度下精制孔鳐软骨多糖抗氧化能力均大于粗提多糖.精制多糖的血管生成抑制活性与硫酸软骨素(CS)相当,并且呈剂量依赖关系.结论:微波辅助碱液提取和木瓜蛋白酶脱蛋白所得精制多糖具有很好的抗氧化和血管生成抑制活性.
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| 关 键 词： | 孔鳐软骨 多糖 微波辅助提取 抗氧化 血管生成抑制活性 |
Microwave-assisted Extraction of the Polysaccharide with Antioxidant and Anti-angiogenesis Activity from the Cartilage of Raja Porosa |
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| Luo Hongyu,Wang Bin,Feng Gang,Qu Youle. Microwave-assisted Extraction of the Polysaccharide with Antioxidant and Anti-angiogenesis Activity from the Cartilage of Raja Porosa[J]. Journal of Chinese Institute of Food Science and Technology, 2011, 11(7) |
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| Authors: | Luo Hongyu Wang Bin Feng Gang Qu Youle |
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| Affiliation: | Luo Hongyu1,2 Wang Bin2 Feng Gang2 Qu Youle2(1School of Life Science and Biotechnology,Huazhong University of Science and Technology,Wuhan 430074 2School of Food and Pharmacy,Zhejiang Ocean University,Zhoushan 316000,Zhejiang) |
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| Abstract: | Objective:Processing technology and in vitro antioxidant and anti-angiogenesis properties of the polysaccharide from the cartilage of Raja porosa were investigated.Method:The optimal conditions of Microwave-assisted alkaline extraction(MAAE) and papain hydrolysis processes were explored using single-factor experiments and orthogonal array design with the extraction ratio of polysaccharide as index.The antioxidant activities of the polysaccharide were mea-sured through DPPH/hydroxyl radical-scavenging activi... |
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| Keywords: | cartilage of Raja porosa polysaccharide microwave-assisted extraction antioxidant anti-angiogenesis activity |
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