| 水提—醇沉法提取佛手粗多糖的最佳工艺研究 |
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| 引用本文： | 纪耀华,袁湘杰.水提—醇沉法提取佛手粗多糖的最佳工艺研究[J].中国食物与营养,2011,17(11):63-65. |
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| 作者姓名： | 纪耀华 袁湘杰 |
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| 作者单位： | 1. 长春医学高等专科学校,长春,150012
2. 白求恩医科大学制药厂,长春,130000 |
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| 基金项目： | 吉林省教育厅“十一五”科学技术研究项目 |
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| 摘 要： | 目的:确定利用传统方法提取佛手粗多糖的最佳工艺条件。方法:以佛手多糖的含量为指标,以提取时间、提取次数、提取温度、料液比为提取因素,通过单因素试验确定因素水平,利用统计分析方法对提取佛手粗多糖的工艺进行优化研究。结论:水提—醇沉法提取佛手粗多糖的最佳工艺条件是:提取温度95℃,浸提时间2h,料液比1:15,提取次数2次,此方法可以为工业大规模生产佛手多糖提供依据。
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| 关 键 词： | 佛手粗多糖 传统方法 提取工艺 正交试验 统计分析 |
Optimum Technique Research on the Extraction of Bergamot Crude Polysaccharide with Ethanol Subsiding Method |
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| JI Yao-hua,YUAN Xiang-jie.Optimum Technique Research on the Extraction of Bergamot Crude Polysaccharide with Ethanol Subsiding Method[J].Food and Nutrition in China,2011,17(11):63-65. |
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| Authors: | JI Yao-hua YUAN Xiang-jie |
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| Affiliation: | JI Yao-hua1,YUAN Xiang-jie2 (1Changchun Medical College,Changchun 130031,China,2Pharmaceutical Factory of Nerman Bethune University of Medical Science,Changchun 130000,China) |
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| Abstract: | 【Objective】To determine the optimum condition of total sugar content of crude polysaccharide from bergamot.【Method】 Using polysaccharide content of bergamot as indicators,the extraction time,extraction times,extraction temperature,solid to liquid ratio as extraction factors,factor level were determined through single factor experiment,and the extraction optimization of bergamot crude polysaccharide was researched by statistical method.【Conclusion】 The optimum conditions of ethanol subsiding method to extract total sugar content of crude polysaccharides from bergamot were extraction temperature 95℃,extraction time 2h,solid to liquid ratio 1：15,and extraction twice.This method will provide basis for the mass industrial production of bergamot polysaccharide. |
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| Keywords: | bergamot crude polysaccharide traditional method extraction technique orthogonal test statistical analysis |
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