| 用Dot—ELISA方法检测生肉中的沙门菌 |
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| 引用本文： | 韩志辉,张红见.用Dot—ELISA方法检测生肉中的沙门菌[J].中国食品卫生杂志,2011,23(6):553-554. |
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| 作者姓名： | 韩志辉 张红见 |
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| 作者单位： | 青海大学动物医学系,青海 西宁,810016 |
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| 摘 要： | 目的 检测生肉中的沙门菌带菌情况,为预防沙门菌食物中毒提供理论参数.方法 应用Dot-ELISA法对西宁市某屠宰厂85份猪胴体,101份羊胴体和71份牛胴体进行沙门菌的检测,同时用常规分离培养鉴定技术作为对照试验.结果 Dot-ELISA法检出沙门菌阳性率分别为76.47％( 65/85),55.44％ (56/101)和46.48％(33/71)；而常规分离培养鉴定技术检出沙门菌阳性率分别为78.82％( 67/85)、47.52％ (48/101)和43.66％(31/71).两种方法的阳性符合率分别为86.57％、85.71％和81.82％,两种方法在检测中的差异无显著性(P＞0.05).结论 Dot-ELISA法检测沙门菌快速、准确,且与分离培养法阳性符合率较高；生肉中沙门菌带菌现象较为严重,存在一定的食品安全隐患.
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| 关 键 词： | Dot-ELISA 检测 生肉 沙门菌 食品安全 食源性致病菌 |
| 收稿时间： | 2011/4/8 0:00:00 |
Dot-ELISA in the detection of salmonella in raw meat |
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| Han zhihui and Zhang hongjian.Dot-ELISA in the detection of salmonella in raw meat[J].Chinese Journal of Food Hygiene,2011,23(6):553-554. |
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| Authors: | Han zhihui and Zhang hongjian |
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| Affiliation: | Han zhihui,Zhang hongjian(Animal medicine Department of Qinghai University,Qinghai Xining 810016,China) |
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| Abstract: | Objective To detect the carriage of Salmonella in raw meat for the prevention of Salmonella food poisoning.Methods Dot-ELISA was used to detect the contamination of Salmonella from 85 pork,71 beef and 101 mutton raw meat samples,and compared with the routine isolation and culture identification technique in some slaughter houses in Xining.Results The positive rate was 76.47%(65/85) for pork,55.44%(56/101) for beef and 46.48%(33/71) for mutton detected by Dot-ELISA;and the positive rate was 78.82%(67/85),47.... |
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| Keywords: | Dot-ELISA detection raw meat Salmonella food safety foodborne pathogens |
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