| 表达豌豆防御素基因的重组酵母对采后柑橘酸腐病的生物防治 |
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| 引用本文： | 任雪艳,郑晓冬.表达豌豆防御素基因的重组酵母对采后柑橘酸腐病的生物防治[J].中国食品学报,2011,11(6). |
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| 作者姓名： | 任雪艳 郑晓冬 |
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| 作者单位： | 1. 浙江大学生物系统工程与食品科学学院,食品科学与营养系,杭州310029;石河子大学生命科学学院,新疆石河子832003
2. 浙江大学生物系统工程与食品科学学院,食品科学与营养系,杭州310029 |
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| 基金项目： | 十一五国家科技支撑计划重点项目(No.2009badb9b09) |
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| 摘 要： | 抗菌肤(Antimicrobial peptides,APMs)是一类对细菌、真菌等微生物及某些昆虫和动植物细胞具有抑制作用的小分子多肽.将碗豆防御素基因Psd1插入甲醇酵母Pichia pastoris的分泌表达质粒pPIC9K中,并将含有目的基因的重组质粒电转化P.pastoris GS115 (his4),得到重组酵母GS 115/PSD菌株.试验结果表明,重组酵母菌株经扩大培养,诱导表达的菌体被破碎后,酵母悬浮液含量达到5×108细胞/mL时,可以很好地抑制采后柑橘的酸腐病.
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| 关 键 词： | 抗菌肽 酸腐 毕赤酵母 |
Biological Control of Postharvest Citrus Sour Rot by Pichia pastoris Recombinant Strains Expressing Pea Defensin Gene |
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| Ren Xueyan,Zheng Xiaodong.Biological Control of Postharvest Citrus Sour Rot by Pichia pastoris Recombinant Strains Expressing Pea Defensin Gene[J].Journal of Chinese Institute of Food Science and Technology,2011,11(6). |
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| Authors: | Ren Xueyan Zheng Xiaodong |
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| Affiliation: | Ren Xueyan1,2 Zheng Xiaodong1*(1Department of Food Science and Nutrition,School of Bio-systems Engineering and Food Science,Zhejiang University,Hangzhou 3100292School of Bio-science,Shihezi University,Shihezi 832003,Xinjiang) |
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| Abstract: | The Antimicrobial peptides are a kinds of small molecular poly peptides which can restrain bacterium,fungi,insect cells,plant cells and animal cells.In this research,the pea defense Psd1 was inserted into the Plasmid pPIC9K of Pichia pastoris to obtain secretory plasmid pPIC-PSD,recombinant yeast strain GS115/PSD was obtained after electroporation transfering this recombinant plasmid to Pichia pastoris GS115(his4),and pick up some high-copy transformants.The study demonstrates that the yeast strain GS115/PS... |
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| Keywords: | antifungal peptide sour rot Pichia pastoris |
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