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| 多重PCR同时检测常见8种食物过敏原 | |
| 引用本文： | 王玮,韩建勋,袁飞,吴亚君,陈颖,葛毅强.多重PCR同时检测常见8种食物过敏原[J].中国食品学报,2011,11(6). |
| 作者姓名： | 王玮 韩建勋 袁飞 吴亚君 陈颖 葛毅强 |
| 作者单位： | 1. 中国检验检疫科学研究院,北京100123;中国农业大学食品学院,北京100083 2. 中国检验检疫科学研究院,北京,100123 3. 中国农业大学食品学院,北京100083;中国农村技术开发中心,北京100045 |
| 基金项目： | 国家自然科学基金项目(No.31071552); 国家“十一五”科技支撑计划项目(No.2009BADB9B03) |
| 摘 要： | 依据大豆Lectin基因,小麦Gliadin基因,花生Arah3基因,腰果Ana o3基因,鱼和虾16S rRNA,牛和鸡线粒体DNA设计特异性引物序列.在单一PCR方法基础上,建立2种4重PCR方法检测8种食物过敏原的技术.该方法检测周期短,具有较好的特异性和灵敏性,可用于对食品中多种食物过敏原的检测和监控. |
| 关 键 词： | 多重PCR 食物过敏原 检测 |
Detection of Eight Food Allergens with Multiplex PCR |
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| Wang Wei,Han Jianxun,Yuan Fei,Wu Yajun,Chen Ying,Ge Yiqiang.Detection of Eight Food Allergens with Multiplex PCR[J].Journal of Chinese Institute of Food Science and Technology,2011,11(6). | |
| Authors: | Wang Wei Han Jianxun Yuan Fei Wu Yajun Chen Ying Ge Yiqiang |
| Affiliation: | Wang Wei1,2 Han Jianxun1 Yuan Fei1 Wu Yajun1 Chen Ying1 Ge Yiqiang2,3*(1Institute of Food Safety,Chinese Academy of Inspection and Quarantine,Beijing 1001232College of Food Science & Nutritional Engineering,China Agricultural University,Beijing 1000833China Rural Technology Development Center,Beijing 100045) |
| Abstract: | Eight pairs of primers were selected according to the sequence of Lectin gene from soybean,Gliadin gene from wheat,Ara h 3 gene from peanut,Ana o3 gene from cashew,16S rRNA from shrimp and fish,mitochondrial DNA from bovine and chicken respectively,and a multiplex PCR assay for food allergen detection was established on the basis of simple PCR technique.Eight food allergens could be detected rapid,specific and sensitive with 2 multiplex PCR assays at the same time.It could be used for detection and surveill... |
| Keywords: | multiplex PCR food allergens detection |
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