| 凝胶过滤分离纯化酪蛋白糖巨肽的研究 |
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| 引用本文： | 袁永俊,张秀媛,胡婷.凝胶过滤分离纯化酪蛋白糖巨肽的研究[J].西华大学学报(自然科学版),2010,29(2):195-198. |
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| 作者姓名： | 袁永俊 张秀媛 胡婷 |
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| 作者单位： | 西华大学生物工程学院,四川,成都,610039 |
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| 基金项目： | 四川省教育厅重点项目资助 |
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| 摘 要： | 酪蛋白糖巨肽具有重要生理功能,可广泛地应用于保健食品和药品。以Superdex 75 10/300 GL为分离介质,对酪蛋白的凝乳酶水解物中的酪蛋白糖巨肽进行分离纯化研究,结果表明,分离纯化CGMP的适宜条件为:0.1M醋酸铵平衡上样、洗脱,洗脱流速0.5ml/min;酶解上清液浓缩25倍上样,上样体积0.5ml。分离所得的CGMP由分子量分别为43000Da、30000Da和25000Da,糖基化程度分别为71.6μg/mg、99.1μg/mg和44.4μg/mg的三个组分组成,其平均糖基化程度为76μg/mg,总蛋白回收率为1.45%。
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| 关 键 词： | 酪蛋白糖巨肽 凝胶过滤 分离纯化 |
Study on the Separation and Purification of Casein Glycomacropeptide by Gel Filtration Chromatography |
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| YUAN Yong-jun,ZHANG Xiu-yuan,HU Ting.Study on the Separation and Purification of Casein Glycomacropeptide by Gel Filtration Chromatography[J].Journal of Xihua University:Natural Science Edition,2010,29(2):195-198. |
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| Authors: | YUAN Yong-jun ZHANG Xiu-yuan HU Ting |
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| Affiliation: | YUAN Yong-jun,ZHANG Xiu-yuan,HU Ting(School of Bioengineering,Xihua University,Chengdu 610039 China) |
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| Abstract: | Casein glycomacropeptide(CGMP) can be widely used in the function food and medicine due to its important physiological action.The authors studied the separation and purification conditions for CGMP from rennase casein hydrolysate by using Superdex 75 10/300 GL as media.The suitable conditions of separation and purification for CGMP are as follows: Superdex 75 10/300 GL is equilibrated and added samples and eluted with 0.1M ammonium acetate buffer,eluted velocity is 0.5ml/min,added volume of samples is 0.5ml... |
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| Keywords: | Casein Glycomacropeptide Gel filtration chromatography Separation and Purification |
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