Migration of DNA molecules through entropic trap arrays: a dissipative particle dynamics study |
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Authors: | E Moeendarbary T Y Ng H Pan K Y Lam |
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Affiliation: | (1) School of Mechanical and Aerospace Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore, 639798, Singapore; |
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Abstract: | Dissipative particle dynamics (DPD) simulations of worm-like chain bead-spring models are used to explore the electrophoresis
migration of DNA molecules traveling through narrow constrictions. The DPD is a relatively new numerical approach that is
able to fully incorporate hydrodynamic interactions. Two mechanisms are identified that cause the size-dependent trapping
of DNA chains and thus mobility differences. First, small molecules are found to be trapped in the deep region due to higher
Brownian mobility and crossing of electric field lines. Second longer chains have higher probability to form hernias at the
entrance of the gap and can pass the entropic barrier more easily. Consequently, longer DNA molecules have higher mobility
and travel faster than shorter chains. The present DPD simulations show good agreement with existing experimental data as
well as published numerical data. |
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