Radio frequency plasma polymer coatings for affinity capture MALDI mass spectrometry |
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Authors: | Li Meiling Timmons Richard B Kinsel Gary R |
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Affiliation: | Department of Chemistry and Biochemistry, University of Texas at Arlington, Arlington, TX 76019-0065, USA. |
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Abstract: | Surface modification of MALDI probes is an attractive approach for combining bioaffinity isolation of targeted biomolecules with mass spectrometric analysis of the captured species. In this work, we demonstrate that a polymer thin film, produced by pulsed rf plasma polymerization of allylamine and deposited directly on a MALDI probe, can be subsequently biotinylated to develop a bioaffinity capture MALDI probe. The synthesis and characterization of the probe by XPS, FT-IR, and AFM is described, and the selective isolation of avidin from a three-component mixture of avidin, lysozyme, and cytochrome c is presented. These initial results offer encouragement for the further exploration of rf plasma polymer deposition as a novel approach for the development of on-probe affinity capture MALDI probes. |
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