松乳菇多糖刺激免疫细胞增殖及诱导肿瘤细胞凋亡的研究

为研究松乳菇多糖(Lactarius deliciosus Gray polysaccharide,LDG-A)对特异性免疫细胞的增殖效应活性及对肿瘤细胞凋亡的影响,在LDG-A作用下检测T淋巴细胞、B淋巴细胞的增殖效应,并观察T淋巴细胞、B淋巴细胞的细胞形态,检测T淋巴细胞、B淋巴细胞在LDG-A的作用下处于细胞周期各个时期的细胞数量及B淋巴细胞分泌抗体的情况,观察LDG-A作用于肺癌细胞A549的凋亡和细胞骨架情况。结果表明:随着LDG-A质量浓度的增加,能够促进T淋巴细胞、B淋巴细胞的增殖,并呈现出一定的剂量依赖关系;同时LDG-A能够减少T淋巴细胞、B淋巴细胞在G0/G~1期的细胞数量百分比,促进细胞进入G~2/M期,具有促进B淋巴细胞分泌抗体免疫球蛋白M、免疫球蛋白D和免疫球蛋白E的作用。在12.5~50.0μg/m L范围内,LDG-A能够诱导肺癌细胞A549凋亡,并呈现出一定的剂量依赖关系;在细胞凋亡时F-肌动蛋白丝断裂,蛋白纤维网络结构被破坏。综上所述,在体外实验中,LDG-A对特异性免疫细胞具有促增殖效应活性,能够促进肿瘤细胞的凋亡。

Polysaccharide from Lactarius deliciosus Gray Stimulates Immunocyte Proliferation and Induces Tumor Cells Apoptosis

To understand the effect of Lactarius deliciosus Gray polysaccharide (LDG-A) on the proliferation of specific immunocytes and tumor cell apoptosis, we tested and observed the proliferation and morphological changes of T lymphocytes and B lymphocytes in the presence of LDG-A, determined the number of T lymphocytes and B lymphocytes in each stage of the cell cycle, and also evaluated antibody secretion by B lymphocytes under the stimulation of LDG-A. In addition, the apoptosis and cytoskeleton of lung cancer cell A549 were evaluated. The results showed that the proliferation of B lymphocytes and T lymphocytes’ proliferation was promoted LDG-A in a dose-response manner. LDG-A could also reduce the number of B lymphocytes at the G0/G1 phase and promote them to enter the G2/M phase. Meanwhile, LDG-A also promoted the secretion of IgM, IgD and IgE by B lymphocytes. In addition, LDG-A in the range of 12.5–50.0 μg/mL could induce the apoptosis of lung cancer cell A549 in a dose-dependent manner. Moreover, we observed fractured F-actin filaments in the cells as well as damaged protein fiber networks during apoptosis. In conclusion, LDG-A has a promoting effect on the proliferation of specific immunocytes, and accelerates the apoptosis of tumor cells in vitro.