1株产蓝色素真菌F10的ITS序列及其色素性质分析

从赣南野生松木上分离到1株产蓝色素真菌F10,该菌经鉴定为葡萄糖座科Pseudofusicoccum属。为研究F10产生的蓝色素的性质,实验采用不同pH值、温度、光照、金属离子、氧化剂和还原剂处理蓝色素,结果表明,该蓝色素对光照、温度、氧化剂都不敏感,对pH值、金属离子、还原剂敏感,推测这几种因素可能改变了色素的化学结构。为确定该蓝色素的结构成分,采用高效液相色谱串联质谱、红外光谱法对其进行鉴定,结果表明该色素为芳香族羧酸类化合物。

Identification of a Fungal Strain F10 Producing Blue Pigment and Properties of the Pigment

A fungal strain producing blue pigment, named F10, was isolated from a wild pine in southern Jiangxi, and it was identified as a member of the genus Pseudofusicoccum in the family Botryosphaeria by using ITS rDNA sequence homology comparison as well as morphological traits. We studied the stability of blue pigment when exposed to different solvents, pH values, temperatures illumination intensities, metal ions, oxidizers and reductants. The data showed that the pigment was sensitive to pH, metal ions, and reductants but not light, temperature or oxidants. We speculated that pH, metal ions, and reductants might change the structure of the pigment. Analysis of the basic chemical structure by high performance liquid chromatography-mass spectrometry (HPLC-MS) and infrared spectroscopy (IR) revealed that the pigment was aromatic carboxylic acid compounds.