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双歧杆菌细菌素Bifidocin A对金黄色葡萄球菌的抑菌作用及其机制
引用本文:刘国荣,郜亚昆,王 欣,刘玉洁,曹季林,王成涛. 双歧杆菌细菌素Bifidocin A对金黄色葡萄球菌的抑菌作用及其机制[J]. 食品科学, 2017, 38(17): 1-7. DOI: 10.7506/spkx1002-6630-201717001
作者姓名:刘国荣  郜亚昆  王 欣  刘玉洁  曹季林  王成涛
作者单位:1.北京食品营养与人类健康高精尖创新中心,北京市食品添加剂工程技术研究中心,北京工商大学,北京 100048;2.黑龙江八一农垦大学食品学院,黑龙江 大庆 163319
基金项目:国家自然科学基金面上项目(31671832);北京市属高校高水平教师队伍建设支持计划青年拔尖人才培育计划项目(CIT&TCD201704034);国家自然科学基金青年科学基金项目(31201407)
摘    要:Bifidocin A是由Bifidobacterium animalis BB04代谢合成的一种新型广谱高效细菌素。以革兰氏阳性的金黄色葡萄球菌为测试敏感菌,分析细菌素Bifidocin A的最低抑菌浓度及不同质量浓度下的抑菌效果,并从敏感菌细胞形态与结构、细胞膜的通透性、细胞膜的完整性以及细胞膜质子移动势的变化4个角度分别探讨该细菌素的抑菌作用机制。结果表明,细菌素Bifidocin A对金黄色葡萄球菌CVCC 26112的最低抑菌浓度为0.058μg/m L,抑菌活性较强且存在浓度依赖性;并初步推测其抑菌作用机制是通过耗散细胞膜质子移动势,增加细胞膜通透性,形成孔洞,进而破坏细胞膜完整性,并最终瓦解细胞。

关 键 词:细菌素  双歧杆菌  金黄色葡萄球菌  抑菌活性  抑菌机制  

Antibacterial Activity and Mechanism of Bifidocin A against Staphylococcus aureus
LIU Guorong,GAO Yakun,WANG Xin,LIU Yujie,CAO Jilin,WANG Chengtao. Antibacterial Activity and Mechanism of Bifidocin A against Staphylococcus aureus[J]. Food Science, 2017, 38(17): 1-7. DOI: 10.7506/spkx1002-6630-201717001
Authors:LIU Guorong  GAO Yakun  WANG Xin  LIU Yujie  CAO Jilin  WANG Chengtao
Affiliation:1. Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing 100048, China; 2. College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China
Abstract:Bifidocin A, produced by Bifidobacterium animalis BB04, is a novel bacteriocin with antimicrobial activity against a wide range of foodborne bacteria. The objective of this study was to investigate the antibacterial activity and mechanism of action of bifidocin A against Staphylococcus aureus. The minimum inhibitory concentration (MIC) of bifidocin A for Staphylococcus aureus CVCC 26112 was 0.058 μg/mL. Time-kill assays showed that bifidocin A effectively inhibited the growth of Staphylococcus aureus CVCC 26112 in a time- and concentration-dependent manner. Results also suggested that bifidocin A exerted its anti-Staphylococcus aureus effect through the dissipation of the cytoplasmic membrane proton motive force (PMF), increased membrane permeability, and the formation of cell membrane pores, leading to the destruction of membrane integrity and ultimately complete disintegration of the cells.
Keywords:bacteriocin  Bifidobacterium spp.  Staphylococcus aureus  antibacterial activity  antibacterial mechanism  
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