人工模拟鼠伤寒沙门氏菌在绿豆芽中的内化定殖能力及其消毒处理

以绿豆为作用载体,研究鼠伤寒沙门氏菌ATCC14028在不同发芽阶段的绿豆芽中的内化定殖能力及其影响因素,在此基础上,采用紫外照射和化学消毒剂处理两种方法对定殖在绿豆芽中的鼠伤寒沙门氏菌进行消毒处理,探索消除绿豆芽中定殖的鼠伤寒沙门氏菌的有效方法。结果表明:在绿豆发芽前期的4个不同的时段(0~48 h内):吸胀期(G_1,0 h)、萌动期(G_2,0~12 h)、发芽初期(G_3,12~24 h)、发芽期(G_4,24~48 h),分别接种不同浓度的鼠伤寒沙门氏菌ATCC14028(10~2、10~4、10~6、10~8 CFU/m L),鼠伤寒沙门氏菌在豆芽中的内化定殖能力不同,但在绿豆发芽的生长周期(约为144 h)结束时,4个不同接种时段的鼠伤寒沙门氏菌的内化量趋于相同。对绿豆芽中内化定殖的鼠伤寒沙门氏菌的消毒处理结果表明,紫外照射消毒处理对内化定殖的鼠伤寒沙门氏菌有明显的去除效果,而次氯酸钠溶液和硝酸银溶液浸泡处理对内化的鼠伤寒沙门氏菌的去除效果并不明显。

Internalization and Colonization Capacity of Salmonella typhimurium in Artificially Contaminated Mung Bean Sprouts and Its Disinfection

In this investigation, the internalization and colonization capacity of Salmonella typhimurium ATCC14028 in artificially contaminated mung bean sprouts during different germination stages was studied. Furthermore, two different disinfection methods of ultraviolet irradiation and chemical disinfectant treatment were developed to eliminate internalized Salmonella typhimurium effectively. During four different stages (0?48 h) of pre-germination of mung bean namely expansion (G1, 0 h), budding (G2, 0?12 h), early germination (G3, 12?24 h) and germination (G4, 24?48 h), Salmonella typhimurium had different internalization and colonization capacities at different inoculation concentrations (102, 104, 106 and 108 CFU/mL), which, however, tended to be identical at the end of the growth cycle (about 144 h). In addition, our results showed that ultraviolet radiation could effectively eliminate internalized Salmonella typhimurium while dipping in a mixed solution of sodium hypochlorite and silver nitrate solution had no such effect.