文蛤寡肽对小鼠急性肝损伤的保护作用

对文蛤寡肽(Meretrix meretrix oligopeptides,MMO)在小鼠急性肝损伤中的保护作用进行研究。首先通过酶解、超滤、Sephadex G-25柱分离技术从文蛤中分离纯化出具有肝修复作用的文蛤寡肽,经检测该目标肽的氨基酸序列为Gln-Leu-Asn-Trp-Asp。然后建立四氯化碳(CCl_4)致小鼠急性肝损伤模型,测定小鼠肝脏指数;检测小鼠血清谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aspartate aminotransferase,AST)、γ-谷氨酰转移酶(γ-glutamyltransferase,γ-GT)、超氧化物歧化酶(superoxide dismutase,SOD)活力及甘油三酯(triglyceride,TG)、丙二醛(malondialdehyde,MDA)水平;苏木精-伊红染色观察肝脏病理学改变;免疫组化法测定肝组织TNF-α、NF-κB蛋白表达量。结果表明:MMO组与模型组比较,小鼠肝指数显著降低(P0.05);血清中ALT、AST、γ-GT活力显著降低(P0.05),γ-GT活力最高降幅达到47.16%;SOD活力显著升高(P0.05);TG、MDA水平显著降低(P0.05),最高降幅分别达到31.88%和28.83%。;苏木精-伊红染色观察肝组织结构明显好转;TNF-α、NF-κB蛋白表达量显著降低。因此,MMO对CCl_4小鼠急性肝损伤有较为明显的保护作用。

Protective Effect of Meretrix meretrix Oligopeptides on Acute Liver Injury in Mice

The protective effect of Meretrix meretrix oligopeptide (MMO) on CCl4-induced acute liver injury in mice was examined. MMO, with the potential to repair damaged liver, was enzymatically extracted and purified by ultrafiltration and Sephadex G-25 column chromatography, and its amino acid sequence was determined to be Gln-Leu-Asn-Trp-Asp. Then, an acute liver injury model was established by intraperitoneal injection of CCl4 into mice. The liver index of mice was measured, the activities of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), γ-glutamyltransferase (γ-GT) and superoxide dismutase (SOD) were determined, and serum triglyceride (TG) and malondialdehyde (MDA) levels were measured. Liver tissue samples from mice were stained with hematoxylin-eosin (HE) to observe the effect of MMO on liver histopathology. Immunohistochemical staining was used to detect the protein expression of TNF-α and NF-κB in hepatic tissue. The results showed that the MMO group exhibited a decrease in liver index, a reduction in serum ALT, AST and γ-GT activities (by up to 47.16%), an elevation in serum SOD activity, and a decline in serum TG and MDA levels (by up to 31.88% and 28.83%) compared with the model group, all these changes being statistically significant. Mouse liver tissue stained with HE revealed a notable improvement in liver histology. The protein expression of TNF-α and NF-κB in hepatic tissue was significantly decreased by administration of MMO. Therefore, MMO can effectively protect against acute liver injury induced by CCl4 in mice.