牡丹花蕊黄酮对H_2O_2诱导RAW264.7巨噬细胞损伤的保护作用

目的:研究牡丹花蕊黄酮对H_2O_2诱导损伤的RAW264.7巨噬细胞的保护作用。方法:实验分为空白组、H_2O_2损伤组、VC对照组和牡丹花蕊黄酮低、中、高剂量组。应用H_2O_2建立RAW264.7巨噬细胞氧化损伤模型,噻唑蓝法测定细胞存活率,试剂盒法测定细胞和细胞培养液中谷胱甘肽和丙二醛含量及过氧化氢酶、超氧化物歧化酶、乳酸脱氢酶活力。结果:牡丹花蕊黄酮的质量浓度在10～30μg/mL范围内时,可显著促进RAW264.7巨噬细胞的增殖(P0.05)。与H2O2损伤组相比,牡丹花蕊黄酮各剂量组能够提高细胞及细胞培养液中总抗氧化能力,谷胱甘肽过氧化物酶、超氧化物酶、过氧化氢酶活力以及还原型谷胱甘肽含量,降低细胞及细胞培养液中丙二醛含量,提高细胞内乳酸脱氢酶的水平。结论:牡丹花蕊黄酮可以促进RAW264.7巨噬细胞的增殖,提高H_2O_2损伤的RAW264.7巨噬细胞的抗氧化能力,且存在剂量依赖效应。

Protective Effect of Flavonoids from Peony Stamens on Hydrogen Peroxide-Induced Toxicity in RAW264.7 Cells

Objective: The aim of this study was to investigate the protective effect of flavonoids from peony stamens (PSF) against hydrogen peroxide (H2O2)-induced injury in RAW264.7 cells. Methods: This experiment was divided into blank, model, control and low-, medium- and high-dose PSF groups. RAW264.7 cells were injured by H2O2 and the cell viability was measured by methyl thiazolyl tetrazolium assay. The contents of malondialdehyde (MDA) and glutathione (GSH) and the activities of catalase (CAT), superoxide dismutase (SOD) and lactate dehydrogenase (LDH) inside the cells and in the culture medium were determined by commercial kits. Results: The flavonoids at concentrations in the range of 10–30 μg/mL significantly enhanced the proliferation of RAW264.7 cells (P < 0.05). Compared with the model group, the flavonoids at all concentrations could increase total antioxidant capacity (T-AOC), the activities of glutathione peroxidase (GSH-Px), SOD and CAT and GSH content, reduce MDA content in the cultured cells and culture medium and improve LDH activity in the cells. Conclusion: Flavonoids from peony stamens can enhance the proliferation of RAW264.7 cells and protect them against H2O2-induced toxicity in a dose-dependent manner.