巴莲莲子生物碱提取物对CCl_4诱导小鼠肝损伤的预防效果

为检测巴莲莲子生物碱提取物(alkaloids extracted from Ba lotus seed,AEBLS)对CCl_4诱导的小鼠肝损伤的预防作用,以732交换树脂分离纯化的AEBLS为研究对象,对小鼠灌胃低、高剂量(50、100 mg/(kg·d))AEBLS后注射2 m L/kg CCl_4诱导小鼠发生肝损伤,观察小鼠肝指数、血清指标和肝组织中相关m RNA表达的强度。结果显示,AEBLS可以降低肝损伤小鼠的肝质量和肝指数(P0.05);AEBLS可以下调肝损伤小鼠血清中的天冬氨酸氨基转移酶、丙氨酸氨基转移酶、碱性磷酸酶、甘油三酯、总胆固醇、尿素氮、一氧化氮、丙二醛水平和上调白蛋白、超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)水平(P0.05);同时AEBLS还可以下调肝损伤小鼠血清中白介素-6(interleukin-6,IL?6)、IL?12、肿瘤坏死因子-α(tumor necrosis factor-alpha,TNF-α)和γ-干扰素细胞因子水平(P0.05)。实时聚合酶链式反应也证实AEBLS能上调肝损伤小鼠肝组织中Mn-SOD、Cu/Zn-SOD、CAT、GSH-Px、核因子κB抑制蛋白α的m RNA表达和下调核因子κB-p65、诱导型一氧化氮合成酶、环氧化酶-2、TNF-α、IL-1β、IL-6的m RNA表达(P0.05)。由此可见,AEBLS对CCl_4引发的肝损伤有较好的预防作用,且效果接近药物水飞蓟素,具有较好的功能性作用。

Preventive Effects of Alkaloids Extracted from Ba Lotus Seeds on CCl4-Induced Hepatic Injury in Mice

The aim of this study was to determine the preventive effects of alkaloids extracted from Ba lotus seeds (AEBLS) on CCl4-induced hepatic injury in mice. AEBLS was purified using 732 exchange resin and then orally administered to mice. Afterwards, the mice were injected with CCl4 to induce hepatic injury, and liver index, serum biochemical parameters, and the mRNA expression of related genes in hepatic tissue were evaluated. The results showed that AEBLS could reduce liver weight and liver index in mice with hepatic injury. AEBLS could decrease aspartate aminotransferase (AST), aminotransferase (ALT), alkaline phosphatase (ALP), triglyceride (TG), total cholesterol (TC), urea nitrogen (BUN), nitric oxide (NO) and malondialdehyde (MDA) levels and increase albumin (ALB), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) levels of the serum of mice with hepatic injury. Meanwhile, it could also increase serum levels of cytokines such as interleukin-6 (IL?6), IL?12, tumor necrosis factor-alpha (TNF-α) and interferon-γ (IFN-γ). RT-PCR results also confirmed that AEBLS could upregulate Mn-SOD, Cu/Zn-SOD, CAT, GSH-Px and IκB-α mRNA expression and downregulate NF-κB-p65, iNOS, COX-2, TNF-α, IL-1β and IL-6 mRNA expression in the liver tissue of mice with hepatic injury. Therefore, AEBLS has a good preventive effect on CCl4-induced hepatic injury, which is close to that of silymarin.