| 植物乳杆菌天然质粒分类 |
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| 引用本文： | 孙大庆,李洪飞,宋大巍,杨建.植物乳杆菌天然质粒分类[J].食品科学,2017,38(12):69-74. |
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| 作者姓名： | 孙大庆 李洪飞 宋大巍 杨建 |
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| 作者单位： | 1.黑龙江八一农垦大学 国家杂粮工程技术研究中心，黑龙江?大庆 163319；2.黑龙江八一农垦大学食品学院，黑龙江?大庆 163319 |
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| 基金项目： | 黑龙江省青年科学基金项目（QC2014C020） |
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| 摘 要： | 为建立植物乳杆菌天然质粒分类方法,以质粒复制起始蛋白(replication initiation protein,Rep)作为分类标记,通过系统进化树分析方法,将植物乳杆菌53个编码Rep天然质粒划分为6个质粒类型,包括5个质粒家族和1个新的复制类型质粒,之后进一步提出了每个质粒家族特有的骨干序列,最终建立了一种简单、有效的植物乳杆菌天然质粒的分类方法和标准。与以往质粒功能和不相容性分类方法相比较,本方法具有较好的实用性和通用性。
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| 关 键 词： | 植物乳杆菌 质粒 基因组 复制起始蛋白 复制子 |
Classification of Natural Plasmids in Lactobacillus plantarum |
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| SUN Daqing,LI Hongfei,SONG Dawei,YANG Jian.Classification of Natural Plasmids in Lactobacillus plantarum[J].Food Science,2017,38(12):69-74. |
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| Authors: | SUN Daqing LI Hongfei SONG Dawei YANG Jian |
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| Affiliation: | 1. National Coarse Cereals Engineering Research Center, Heilongjiang Bayi Agricultural University, Daqing 163319, China; 2. College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China |
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| Abstract: | This study aimed to establish a classification method for natural plasmids of Lactobacillus plantarum (L. plantarum) by using plasmid replication initiation protein (Rep) as a taxonomic marker. Fifty three plasmids encoding Rep in L. plantarum were divided into six plasmid types by the method of phylogenetic analysis, including five plasmid families and one novel replication type of plasmid. Then, the backbone sequence specific to each plasmid family was further proposed. Finally, a simple and effective classification method and criterion for L. plantarum natural plasmids was established. Compared with the previous classification methods based on plasmid function and incompatibility, this method displayed relatively better practicability and versatility. |
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| Keywords: | Lactobacillus plantarum plasmid genome replication initiation protein replicon |
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