| 基于2种衍生化方法对牛乳中寡糖的高效液相色谱-质谱联用分析 |
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| 引用本文： | 陈新新,芦 晶,刘 鹭,逄晓阳,张书文,李 诚,吕加平.基于2种衍生化方法对牛乳中寡糖的高效液相色谱-质谱联用分析[J].食品科学,2017,38(10):162-167. |
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| 作者姓名： | 陈新新 芦 晶 刘 鹭 逄晓阳 张书文 李 诚 吕加平 |
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| 作者单位： | 1.四川农业大学食品学院，四川 雅安 625014；2.中国农业科学院农产品加工研究所，北京 100193 |
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| 基金项目： | 国家自然科学基金青年科学基金项目（1401514）；“十二五”国家科技支撑计划项目（2013BAD18B10）；
公益性行业（农业）科研专项（201303085） |
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| 摘 要： | 利用Sep-Pak C_(18)柱和石墨化碳柱对已脱脂和除去蛋白质的牛乳进行分离纯化,并采用1-苯基-3-甲基-5-唑啉酮(3-methyl-1-phenyl-2-pyrazolin-5-one,PMP)和2-氨基吖啶酮(2-aminoacridone,AMAC),2种衍生化试剂对其进行衍生化处理,通过高效液相色谱进行优化,AMAC衍生化的结果(约有67个峰)明显优于PMP衍生化的结果(约20个峰),最终确定AMAC为最佳衍生化试剂,并利用最终优化好的高效液相色谱条件,采用高效液相色谱-串联离子阱飞行时间质谱对牛乳中寡糖进行测定,最终测得7种乳寡糖并获得该7种乳寡糖的单糖组成。
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| 关 键 词： | 牛乳寡糖 1-苯基-3-甲基-5-唑啉酮 2-氨基吖啶酮 高效液相色谱-串联离子阱飞行时间质谱 |
Comparative Analysis of Oligosaccharides in Milk Based on Two Different Derivatization Methods by
HPLC-Tandem Mass Spectrometry |
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| CHEN Xinxin,LU Jing,LIU Lu,PANG Xiaoyang,ZHANG Shuwen,LI Cheng,Lü Jiaping.Comparative Analysis of Oligosaccharides in Milk Based on Two Different Derivatization Methods by
HPLC-Tandem Mass Spectrometry[J].Food Science,2017,38(10):162-167. |
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| Authors: | CHEN Xinxin LU Jing LIU Lu PANG Xiaoyang ZHANG Shuwen LI Cheng Lü Jiaping |
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| Affiliation: | 1. College of Food Science, Sichuan Agricultural University, Ya’an 625014, China;
2. Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China |
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| Abstract: | In the present study, Sep-Pak C18 and porous graphitized carbon column chromatographies were sequentially
used to separate and purify oligosaccharides from defatted and deproteinated milk. After derivatization with phenyl-3-
methyl-5-pyrazolone (PMP) or 2-aminoacridone (AMAC), the oligosaccharides were analyzed by high-performance liquid
chromatography ion trap time of flight tandem mass spectrometry (HPLC-IT-TOF-MS). AMAC was a better derivatization agent
than PMP, since they produced about 67 and 20 chromatographic peaks, respectively. Finally, seven lacto-oligosaccharides were
identified under optimized HPLC conditions, and their monosaccharide compositions were determined. |
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| Keywords: | milk oligosaccharides 3-methyl-1-phenyl-2-pyrazolin-5-one 2-aminoacridone high-performance liquid
chromatography ion trap time of flight tandem mass spectrometry (HPLC-IT-TOF-MS) |
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