‘紫娟’茶花色苷的分离鉴定

依次以MCI gel CPH 20P(75~150μm)树脂和Sephadex~(TM) LH-20葡聚糖凝胶为层析柱填料,对‘紫娟’茶花色苷进行分离纯化,采用5%乙酸-甲醇溶液和5%乙酸溶液对花色苷提取液梯度洗脱,得到6种花色苷组分。采用薄层层析、高效液相色谱及高效液相色谱-电喷雾-串联质谱对‘紫娟’茶花色苷组成成分进行研究。结果表明:从‘紫娟’茶鲜叶中分离出的花色苷为飞燕草素-3-O-半乳糖苷、矢车菊素-3-O-半乳糖苷、飞燕草素-3-O-(6-(Z)对香豆酸)吡喃半乳糖苷、矢车菊素-3-O-(6-(Z)对香豆酸)吡喃半乳糖苷、飞燕草素-3-O-(6-(E)对香豆酸)吡喃半乳糖苷、矢车菊素-3-O-(6-(E)对香豆酸)吡喃半乳糖苷。

Separation and Identification of Anthocyanins from ‘Zijuan’ Tea Leaves

The anthocyanins from leaves of the tea cultivar ‘Zijuan’ were separated and purified by sequential column chromatography using MCI gel CHP 20P resin (75–150 μm) and SephadexTM LH-20, through gradient elution with 5% acid-methanol and 5% acid-water. A total of 6 anthocyanins were separated from the purified extract, which were analyzed by high performance liquid chromatography (HPLC), thin layer chromatography (TLC) and HPLC-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS). The results showed that the anthocyanins were identified as delphinidin 3-O-β-D-galactoside, cyanidin 3-O-β-D-galactoside, delphinidin 3-O-β-D-(6-(Z)-p-coumaroyl)galactopyranoside, cyanidin 3-O-β-D-(6-(Z)-p-coumaroyl)galactopyranoside, delphinidin 3-O-β-D-(6-(E)-p-coumaroyl)galactopyranoside and cyanidin 3-O-β-D-(6-(E)-p-coumaroyl)galactopyranoside.