表面等离子体共振生物芯片无标记实时检测虾血蓝蛋白

利用自行研制的便携式表面等离子体共振生物芯片检测系统,在生物芯片表面分别固定虾血蓝蛋白、虾血蓝蛋白的单克隆抗体、虾血蓝蛋白的单克隆抗体腹水作为生物探针,利用免疫反应的特异性,研究虾血蓝蛋白与其单克隆抗体的相互作用,分析动力学反应过程,建立标准曲线。用同一个生物芯片检测了8个抗体样品、7个未纯化的抗体腹水,为现场检测大量食品中过敏原、检测临床血清中过敏原特异性抗体进行基础研究。表面等离子体共振生物芯片检测过敏原,仪器便携、操作简便、无需标记、无污染、成本低,可进行现场大量样品的实时连续检测和快速筛选,适用于超市、集市、工厂等需要实时快速检测和质量监控的场所,也可以应用于临床上患者血清样品的过敏原抗体检测。

A Label-Free Surface Plasmon Resonance Biochip for in Situ Detection of Shrimp Hemocyanin

In this paper, a rapid label-free method to analyze the immune response to allergens by using a portable surface plasmon resonance (SPR) biochip was presented. Shrimp hemocyanin, monoclonal antibody against shrimp hemocyanin, and ascites fluid containing the monoclonal antibody were immobilized onto the surface of the biochip as probes, respectively. The biochip was then used to detect the immune response between shrimp hemocyanin and the corresponding monoclonal antibody. The kinetics of the reaction was analyzed, and the standard curve was constructed. Eight different batches of samples and seven different batches of unpurified ascites were detected on the same chip. This method allowed real-time, label-free, low-cost, environment-friendly and easy-to-control detections. It was suitable for real-time and continuous detection and rapid screening of a large number of samples. The developed portable SPR biochip detection system can be widely applicable to real-time rapid detection and quality control in factories, markets, supermarkets and other fields. It can also be applied in the clinical detection of allergen-specific antibody in serum of patients.