野生嗜杀白假丝酵母LFA418的产毒条件优化及其毒素粗提物特性

对1株分离自新疆楼兰葡萄酒厂野生白假丝酵母(Candida albicans)LFA418的嗜杀特征及其粗毒素特性进行研究。结果显示,C.albicans LFA418具有致死克鲁维毕赤酵母(Pichia kluyveri)、贝酵母(Saccharomyces bayanus)、Lachancea thermotolerans和葡萄牙假丝酵母(Candida lusitaniae)的嗜杀性。接种体积分数7%C.albicans LFA418到YEPD液体培养基中,25℃培养32 h,可以获得最大产量的嗜杀毒素;利用截留分子质量为8 000 D的透析袋制备的粗毒素,在温度分别-20、4℃时pH 4.2~4.6和pH 4.2时温度4~20℃具有嗜杀活性的稳定性。

Optimization of Culture Conditions for Toxin Production by Wild Killer Yeast Candida albicans LFA418

The killer property Candida albicans LFA418, a wild strain isolated from a winery in Loulan of Northwest China’sXinjiang region. The killer feature and crude killer toxin characteristics of a wild yeast strain Candida albicans isolated from Loulan winery of Xinjiang were studied. The results indicated that Candida albicans LFA418 had a wide spectrum of killering activity against Pichia kluyveri, Saccharomyces bayanus, Lachancea thermotolerans and Candida lusitaniae. The optimal culture conditions to obtain the maximum yield of killer toxins were as follows: inoculums of C. albicans LFA418, 7% (V/V); temperature, 25 ℃, and culture time, 32 h. The activity of crude killer toxins was stable at pH 4.2-4.6 when temperature was -20 ℃ and 4 ℃ and temperature 4-20 ℃ at pH 4.2.