| 高压液相色谱法测定那他霉素的含量 |
| |
| 引用本文： | 刘月,王娅莉,张惠敏,于兰,安兰兰. 高压液相色谱法测定那他霉素的含量[J]. 河北化工, 2013, 0(8): 129-130 |
| |
| 作者姓名： | 刘月 王娅莉 张惠敏 于兰 安兰兰 |
| |
| 作者单位： | 华北制药集团新药研究开发有限责任公司,微生物药物国家工程研究中心,河北省工业微生物代谢工程技术研究中心,河北石家庄050015 |
| |
| 摘 要： | 采用高压液相色谱法测定那他霉素的含量，以C18柱（4．6mm×250mm，5μm）为分析柱，以醋酸铵缓冲液（取醋酸铵3g，加水760mL）-乙腈（760：240）为流动相，流速为1．0mL/min，柱温为45cc，检测波长为303nm。线性范围为0．04-0．3mg／mL，线性方程为y=52196216x＋202922，r=1．000，加样回收率为99．73％，RSD为0．74％。该方法简单、准确、灵敏、重复性好。
|
| 关 键 词： | 高压液相色谱法 那他霉素 抗生素 |
Determination of Natamycin by High Performance Liquid Chromatography |
| |
| LIU Yue,WANG Ya-li,ZHANG Hui-min,YU Lan,AN Lan-lan. Determination of Natamycin by High Performance Liquid Chromatography[J]. Hebei Chemical Engineering and Industry, 2013, 0(8): 129-130 |
| |
| Authors: | LIU Yue WANG Ya-li ZHANG Hui-min YU Lan AN Lan-lan |
| |
| Affiliation: | ( NCPC New Drug Recearch and Development Corporation, Ltd., National Engineering Research Center of Microbial Medicine,Hebei Industry Microbial Engineering and Technology Research Center, Shijiazhuang 050015,China ) |
| |
| Abstract: | An HPLC method for determination of natamycin was established. A Cls column（4.6 mm × 250 mm, 5 μm） was used with the phase of ammonium trile （760 ： 240）, at the detection wavelength of 303 nm and the column temperature of 45 ℃ .The calibration curves of natamycin were liner in the concentration rages of 0.04-0.3 mg/mL. The regression equation was y = 52 196 216x ＋ 202 922, r = 1.000.The recovery was 99.73% with RSD 0.74%. The method was simple, accurate and reproducible. |
| |
| Keywords: | high performance liquid chromatography natamycin antibiotic |
| 本文献已被 维普 等数据库收录！ |
|
