| 含~3H标记物的生物样品的制备与测量 |
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| 引用本文： | 沈文梅,田增悦,刘福陆,唐秀兰,王太行,苏妙根.含~3H标记物的生物样品的制备与测量[J].核技术,1986(6). |
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| 作者姓名： | 沈文梅 田增悦 刘福陆 唐秀兰 王太行 苏妙根 |
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| 作者单位： | 军事医学科学院放射医学研究所
(沈文梅，田增悦，刘福陆，唐秀兰，王太行)，中国科学院上海生物化学研究所(苏妙根) |
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| 摘 要： | 在液体闪烁技术中,消化法与燃烧法制备的样品同属均相测量。我们曾用HClO_4-H_2O_2消化制备含~3H的生物样品,遇到了测量效率过低、各种来源样品间的淬灭度相差较大等问题,后来采用了氧瓶燃烧法解决了这个问题。由于药物代谢研究中每批实验取自各种来源的生物样品很多,为此,我们结合本实验室条件,建立了一种用燃烧法制备含~3H标记物的生物样品,在LKB1215型液体闪烁仪上测量放射性的规范化操作程序。
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| 关 键 词： | 生物样品的~3H标记物 燃烧法 药物代谢 |
Preparation of ~3H-labelled biological samples for counting |
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| Shen Wenmei Tian Zengyue Liu FuluTang Xiulan Wang Taihang.Preparation of ~3H-labelled biological samples for counting[J].Nuclear Techniques,1986(6). |
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| Authors: | Shen Wenmei Tian Zengyue Liu FuluTang Xiulan Wang Taihang |
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| Abstract: | A combustion method for preparation of 5H-labelled biological samples for counting was reported. The recovery of this 'method was about 94-97%, and the efficiency of counting was 32-37%. The error due to quenching was reduced to a much lower degree. This method was successfully used in the study of drug metabolism. |
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| Keywords: | 3H-labelled compounds in biological samples combustion method drug metabolism |
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