Alterations in Protein Expression of Arabidopsis thaliana Cell Cultures During Hyper- and Simulated Micro-Gravity |
| |
Authors: | Žarko Barjaktarović Maren Babbick Alfred Nordheim Tobias Lamkemeyer Elisabeth Magel Rüdiger Hampp |
| |
Affiliation: | 1. Physiological Ecology of Plants, Botany Institute, University of Tübingen, Auf der Morgenstelle 1, 72076, Tübingen, Germany 2. Interfaculty Institute for Cell Biology, University of Tübingen, Proteom Centrum Tübingen, Auf der Morgenstelle 15, 72076, Tübingen, Germany 3. University of Hamburg, Zentrum Holzwirtschaft, Abteilung Holzbiologie, Leuschnerstr. 91, 21031, Hamburg, Germany
|
| |
Abstract: | Callus cell cultures of Arabidopsis thaliana exposed to hypergravity (8×g), 2D clinorotation and random positioning exhibit changes in gene expression (Martzivanou et al., Protoplasma 229:155–162, 2006). In a recent investigation we could show that after 2 h of exposure also the protein complement shows treatment-related changes which are indicative for reactive oxygen species being involved in the perception of/response to changes in the gravitational field. In the present study we have extended these investigations for a period of up to 16 h of exposure. We report on changes in abundance of 28 proteins which have been identified by nano HPLC-ESI-MS/MS, and which were altered in amount after 2 h of treatment. According to changes between 2 and 16 h we could distinguish four groups of proteins which either declined, increased from down-regulated to control levels, showed a transient decline or a transient increase. With regard to function, our data indicate stress relief or adaptation to a new gravitational steady state under prolonged exposure. The latter assumption is supported by the appearance of a new set of 19 proteins which is changed in abundance after 8 h of hypergravity. A comparative analysis of the different treatments showed some similarities in response between 8×g centrifugation and 2D clinorotation, while random positioning showed the least responses. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|