固相萃取- 高效液相色谱检测法测定食用菌中三聚氰胺残留量

建立食用菌中三聚氰胺的固相萃取- 高效液相色谱检测法。样品经三氯乙酸、乙腈提取，离心，混合型固相萃取小柱净化后，过0.45μm 滤膜，用配有二极管阵列检测器(PAD)的液相色谱仪检测，外标法定量。同时，以三聚氰胺标准品进行添加回收率测定，结果显示，本方法对三聚氰胺的测定低限为2.0mg/kg，回收率为81.3%～91.7%，测定的相对标准偏差均不大于5.6%。本方法能满足食用菌中三聚氰胺残留量常规检测的需要。

Determination of Melamine Residue in Edible Fungi by Solid Phase Extraction and High Performance Liquid Chromatography

A novel method based on solid phase extraction followed by high performance liquid chromatography (HPLC) was developed for the analysis of melamine residue in edible fungi. Samples were extracted with a mixed system composed of trichloroacetic acid and acetonitrile (3:1, V/V), cleaned up on mixed-mode SPE column and filtrated through 0.45 μm pore size membrane prior to analysis using a HPLC system equipped with PAD. Melamine was quantified by external standard method. The method exhibited a limit of detection of 2.0 mg/kg and a spike recovery range of 81.3% to 91.7% at three levels, with relative standard deviations (RSDs) of not more than 5.6%. The method proves to be most suitable for the determination of melamine residue in edible fungi.