| 双水相萃取法应用于从白萝卜中快速提取过氧化物酶 |
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| 引用本文： | 黄燕华,贾楠,严岩,李任强.双水相萃取法应用于从白萝卜中快速提取过氧化物酶[J].广东化工,2006,33(1):31-32,41. |
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| 作者姓名： | 黄燕华 贾楠 严岩 李任强 |
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| 作者单位： | 暨南大学,生物工程学系,广东,广州,510632 |
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| 基金项目： | 暨南大学校科研和教改项目;暨南大学校科研和教改项目 |
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| 摘 要： | 基于双水相萃取法在化工分离上的优点,研究了其应用于从白萝卜中快速提取过氧化物酶的可行性。结果表明,相对于传统盐析法,应用聚乙二醇/硫酸铵双水相萃取法从白萝卜中提取过氧化物酶具有快速、操作简单、提取率高、成本低及可以使生产规模化等优点。本研究对于过氧化物酶制剂的工业化生产或开发利用白萝卜等具有重要意义。
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| 关 键 词： | 双水相萃取法 白萝卜 过氧化物酶 纯化 |
| 收稿时间： | 2005-11-20 |
| 修稿时间： | 2005-11-20 |
Application on Aqueous Two-Phase Extraction to Fast Preparation of Peroxidase from Radish |
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| Huang Yanhua,Jia Nan,Yan Yan,Li Renqiang.Application on Aqueous Two-Phase Extraction to Fast Preparation of Peroxidase from Radish[J].Guangdong Chemical Industry,2006,33(1):31-32,41. |
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| Authors: | Huang Yanhua Jia Nan Yan Yan Li Renqiang |
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| Affiliation: | Department of Biotechnology, Jinan University, Guangzhou 510632, China |
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| Abstract: | Based on the advantage of aqueous two-phase extraction(ATPE) on chemical separation,its application to fast preparation of peroxidase(POD) from white turnip has been studied.Results indicated that poly(ethylene glycol)(PEG)/(NH_4)_2SO_4 system was quick and simple in extracting POD from white turnip with higher productivity and lower cost compared to conventional salting out.It was suitable to the practical production. |
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| Keywords: | aqueous two-phase extraction white turnip(Raphanus sativus L ) peroxidase purification |
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